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Chitosan-mediated formation of biomimetic silica nanoparticles: An effective method for manganese peroxidase immobilization and stabilization
Alternative TitleJ. Biosci. Bioeng.
Luan, Pan-Pan1,2; Jiang, Yan-Jun2; Zhang, Song-Ping1,3; Gao, Jing2; Su, Zhi-Guo1; Ma, Guang-Hui1; Zhang, Yu-Fei1
2014-11-01
Source PublicationJOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN1389-1723
Volume118Issue:5Pages:575-582
AbstractOur work here, for the first time, reported the use of chitosan-mediated biomimetic silica nanoparticles in enzyme immobilization. In order to make clear the relationship among silicification process, silica nanoparticle structure and immobilized enzyme activity, a mechanism of chitosan-mediated silicification using sodium silicate as the silica source was primarily evaluated. Chitosan was demonstrated effectively to promote the silicification not only in accelerating the aggregation rate of sodium silicate, but also in templating the formation of silica nanoparticles. Although the whole biomimetic silicification process contained polycondensation-aggregation precipitation three stages, the elemental unit in precipitated silica was confirmed to be nanoparticles with 100 nm diameter regardless of the chitosan and silicate concentration used. Furthermore, the effect of enzyme on silicification process was also investigated. The introducing of manganese peroxidase (MnP) to silica precursor solution had no obvious effect on the silicification rate and nanoparticle morphology. The residual activity and embedding rate of immobilized MnP were 64.2% and 36.4% respectively under the optimum conditions. In addition, compared to native MnP, the MnP embedded in chitosan/silica nanoparticles exhibited improved stability against organic solvent and ultrasonic wave. After ultrasonic treatment for 20 min, 77% of the initial activity was remained due to the protective effect of chitosan/silica nanoparticles, while native MnP lost almost all of its original activity. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.; Our work here, for the first time, reported the use of chitosan-mediated biomimetic silica nanoparticles in enzyme immobilization. In order to make clear the relationship among silicification process, silica nanoparticle structure and immobilized enzyme activity, a mechanism of chitosan-mediated silicification using sodium silicate as the silica source was primarily evaluated. Chitosan was demonstrated effectively to promote the silicification not only in accelerating the aggregation rate of sodium silicate, but also in templating the formation of silica nanoparticles. Although the whole biomimetic silicification process contained polycondensation-aggregation precipitation three stages, the elemental unit in precipitated silica was confirmed to be nanoparticles with 100 nm diameter regardless of the chitosan and silicate concentration used. Furthermore, the effect of enzyme on silicification process was also investigated. The introducing of manganese peroxidase (MnP) to silica precursor solution had no obvious effect on the silicification rate and nanoparticle morphology. The residual activity and embedding rate of immobilized MnP were 64.2% and 36.4% respectively under the optimum conditions. In addition, compared to native MnP, the MnP embedded in chitosan/silica nanoparticles exhibited improved stability against organic solvent and ultrasonic wave. After ultrasonic treatment for 20 min, 77% of the initial activity was remained due to the protective effect of chitosan/silica nanoparticles, while native MnP lost almost all of its original activity. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.
KeywordChitosan Biomimetic Silicification Manganese Peroxidase Silica Nanoparticle Immobilization
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jbiosc.2014.05.003
URL查看原文
Indexed BySCI
Language英语
WOS KeywordCOLLOIDAL SILICA ; DIATOM BIOSILICA ; ORGANIC MATRIX ; IN-VITRO ; SILICIFICATION ; PH ; DEGRADATION ; ANTHRACENE ; SEPARATION ; PEPTIDES
WOS Research AreaBiotechnology & Applied Microbiology ; Food Science & Technology
WOS SubjectBiotechnology & Applied Microbiology ; Food Science & Technology
WOS IDWOS:000347141300015
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11803
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Hebei Univ Technol, Sch Chem Engn & Technol, Tianjin 300130, Peoples R China
3.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China
Recommended Citation
GB/T 7714
Luan, Pan-Pan,Jiang, Yan-Jun,Zhang, Song-Ping,et al. Chitosan-mediated formation of biomimetic silica nanoparticles: An effective method for manganese peroxidase immobilization and stabilization[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2014,118(5):575-582.
APA Luan, Pan-Pan.,Jiang, Yan-Jun.,Zhang, Song-Ping.,Gao, Jing.,Su, Zhi-Guo.,...&Zhang, Yu-Fei.(2014).Chitosan-mediated formation of biomimetic silica nanoparticles: An effective method for manganese peroxidase immobilization and stabilization.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,118(5),575-582.
MLA Luan, Pan-Pan,et al."Chitosan-mediated formation of biomimetic silica nanoparticles: An effective method for manganese peroxidase immobilization and stabilization".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 118.5(2014):575-582.
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