CAS OpenIR  > 研究所(批量导入)
Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain
Alternative TitleBiotechnol. Biotechnol. Equip.
Wang, Honghui1; Pan, Jiachuan2; Wang, Jing1; Wang, Nan1; Zhang, Jie1; Li, Qiang3; Wang, Dan1; Zhou, Xiaohua1
2014-11-02
Source PublicationBIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
ISSN1310-2818
Volume28Issue:6Pages:1042-1049
AbstractSuccinic acid (1,4-butanedioic acid) is identified as one of important building-block chemicals. Xylose mother liquor is an abundant industrial residue in xylitol biorefining industry. In this study, xylose mother liquor was utilized to produce succinic acid by recombinant Escherichia coli strain SD121, and the response surface methodology was used to optimize the fermentation media. The optimal conditions of succinic acid fermentation were as follows: 82.62 g L-1 total initial sugars, 42.27 g L-1 MgCO3 and 17.84 g L-1 yeast extract. The maximum production of succinic acid was 52.09 +/- 0.21 g L-1 after 84h with a yield of 0.63 +/- 0.03 g g(-1) total sugar, approaching the predicted value (53.18 g L-1). It was 1.78-fold of the production of that obtained with the basic medium. This was the first report on succinic acid production from xylose mother liquor by recombinant E. coli strains with media optimization using response surface methodology. This work suggested that the xylose mother liquor could be an alternative substrate for the economical production of succinic acid by recombinant E. coli strains.; Succinic acid (1,4-butanedioic acid) is identified as one of important building-block chemicals. Xylose mother liquor is an abundant industrial residue in xylitol biorefining industry. In this study, xylose mother liquor was utilized to produce succinic acid by recombinant Escherichia coli strain SD121, and the response surface methodology was used to optimize the fermentation media. The optimal conditions of succinic acid fermentation were as follows: 82.62 g L-1 total initial sugars, 42.27 g L-1 MgCO3 and 17.84 g L-1 yeast extract. The maximum production of succinic acid was 52.09 +/- 0.21 g L-1 after 84h with a yield of 0.63 +/- 0.03 g g(-1) total sugar, approaching the predicted value (53.18 g L-1). It was 1.78-fold of the production of that obtained with the basic medium. This was the first report on succinic acid production from xylose mother liquor by recombinant E. coli strains with media optimization using response surface methodology. This work suggested that the xylose mother liquor could be an alternative substrate for the economical production of succinic acid by recombinant E. coli strains.
KeywordResponse Surface Methodology Succinic Acid Fermentation Escherichia Coli Xylose Mother Liquor
SubtypeReview
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1080/13102818.2014.952501
URL查看原文
Indexed BySCI
Language英语
WOS KeywordRESPONSE-SURFACE METHODOLOGY ; ALKALINE PROTEASE PRODUCTION ; ACTINOBACILLUS-SUCCINOGENES ; ANAEROBIC CONDITIONS ; RENEWABLE RESOURCES ; BOVINE RUMEN ; BACILLUS SP ; FERMENTATION ; OPTIMIZATION ; HYDROLYSATE
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000348931600008
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11836
Collection研究所(批量导入)
Affiliation1.Chongqing Univ, Coll Chem & Chem Engn, Chongqing 400044, Peoples R China
2.Syracuse Univ, Dept Biomed & Chem Engn, Syracuse, NY 13244 USA
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Honghui,Pan, Jiachuan,Wang, Jing,et al. Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain[J]. BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,2014,28(6):1042-1049.
APA Wang, Honghui.,Pan, Jiachuan.,Wang, Jing.,Wang, Nan.,Zhang, Jie.,...&Zhou, Xiaohua.(2014).Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain.BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT,28(6),1042-1049.
MLA Wang, Honghui,et al."Succinic acid production from xylose mother liquor by recombinant Escherichia coli strain".BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT 28.6(2014):1042-1049.
Files in This Item:
File Name/Size DocType Version Access License
Succinic acid produc(639KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Honghui]'s Articles
[Pan, Jiachuan]'s Articles
[Wang, Jing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Honghui]'s Articles
[Pan, Jiachuan]'s Articles
[Wang, Jing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Honghui]'s Articles
[Pan, Jiachuan]'s Articles
[Wang, Jing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.