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Improved propionic acid and 5,6-dimethylbenzimidazole control strategy for vitamin B12 fermentation by Propionibacterium freudenreichii
Alternative TitleJ. Biotechnol.
Wang, P.; Zhang, Z. W.; Jiao, Y. J.; Liu, S. X.; Wang, Y. S.
2015
Source PublicationJournal of Biotechnology
IssueJANPages:123-129
AbstractAn efficient fermentation-strengthening approach was developed to improve the anaerobic production of vitamin B12 by cultivation process optimization with Propionibacterium freudenreichit The effects of the byproduct propionic acid and the precursor 5,6-dimethylbenzimidazole (DMB) on vitamin B12 biosynthesis were investigated. Byproduct inhibition experiments showed that maintaining propionic acid concentration in broth below 10-20 g/L in the early stage and 20-30 g/L in the late stage can efficiently improve vitamin B12 biosynthesis. Batch fermentation indicated the occurrence of feed-back inhibition in intracellular intermediate biosynthesis. In addition, the incorporation of the precursor DMB depended on the fermentation level of the vitamin B12 intermediate. High vitamin B12 concentration (58.8 mg/L) and production (0.37 mg/g) were obtained with an expanded bed adsorption bioreactor by using the propionic acid and DMB control method. The optimum concentration and production of 59.5 and 0.59 mg/L h for vitamin B12 production were respectively achieved after five continuous batches. (C) 2014 Elsevier B.V. All rights reserved.
KeywordVitamin B12 Propionic Acid Feed Back 5 Inhibition 6-dimethylbenzimidazole Expanded Bed Adsorption Bioreactor Pseudomonas-denitrificans Wheat-straw Biosynthesis Enzyme
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Indexed BySCI
Language英语
WOS IDWOS:000346826800020
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11844
Collection研究所(批量导入)
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GB/T 7714
Wang, P.,Zhang, Z. W.,Jiao, Y. J.,et al. Improved propionic acid and 5,6-dimethylbenzimidazole control strategy for vitamin B12 fermentation by Propionibacterium freudenreichii[J]. Journal of Biotechnology,2015(JAN):123-129.
APA Wang, P.,Zhang, Z. W.,Jiao, Y. J.,Liu, S. X.,&Wang, Y. S..(2015).Improved propionic acid and 5,6-dimethylbenzimidazole control strategy for vitamin B12 fermentation by Propionibacterium freudenreichii.Journal of Biotechnology(JAN),123-129.
MLA Wang, P.,et al."Improved propionic acid and 5,6-dimethylbenzimidazole control strategy for vitamin B12 fermentation by Propionibacterium freudenreichii".Journal of Biotechnology .JAN(2015):123-129.
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