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A sensitive and rapid immunoassay for mycoplasma pneumonia based on Fe3O4 nanoparticles
Alternative TitleMater. Lett.
Yang, Mingzhu1; Guan, Yueping1; Yang, Yu2; Xia, Tingting1; Xiong, Wubin1; Guo, Chen3
2014-12-15
Source PublicationMATERIALS LETTERS
ISSN0167-577X
Volume137Issue:DEC.Pages:113-116
AbstractThe peroxidase-like activity of dissolved Fe3O4 magnetic nanoparticles (MNPs) was determined and compared with whole Fe3O4 MNPs in this study. We detected the peroxidase-like activity of Fe3O4 MNPs through the catalytic reaction between Fe3O4 MNPs and TMB. Due to the high catalytic activity of ferric ions and ferrous ions released from Fe3O4 MNPs after dissolution, dissolved Fe3O4 MNPs showed much better peroxidase-like activity than whole Fe3O4 MNPs. In addition, we used the peroxidase-like activity of Fe3O4 MNPs in the diagnosis of mycoplasma pneumonia infection in which Fe3O4 MNPs was used as substitution of horseradish peroxidase. Both Fe3O4 MNPs modified with amino groups (MNPs-NH2) and Fe3O4 MNPs modified with polyethyleneimine (MNPs-PEI) were successfully used in the indirect immunoassay for the detection of mycoplasma pneumoniae. And the results of dissolved Fe3O4 MNPs were more sensitive and specific than that of whole Fe3O4 MNPs. (C) 2014 Elsevier B.V. All rights reserved.; The peroxidase-like activity of dissolved Fe3O4 magnetic nanoparticles (MNPs) was determined and compared with whole Fe3O4 MNPs in this study. We detected the peroxidase-like activity of Fe3O4 MNPs through the catalytic reaction between Fe3O4 MNPs and TMB. Due to the high catalytic activity of ferric ions and ferrous ions released from Fe3O4 MNPs after dissolution, dissolved Fe3O4 MNPs showed much better peroxidase-like activity than whole Fe3O4 MNPs. In addition, we used the peroxidase-like activity of Fe3O4 MNPs in the diagnosis of mycoplasma pneumonia infection in which Fe3O4 MNPs was used as substitution of horseradish peroxidase. Both Fe3O4 MNPs modified with amino groups (MNPs-NH2) and Fe3O4 MNPs modified with polyethyleneimine (MNPs-PEI) were successfully used in the indirect immunoassay for the detection of mycoplasma pneumoniae. And the results of dissolved Fe3O4 MNPs were more sensitive and specific than that of whole Fe3O4 MNPs. (C) 2014 Elsevier B.V. All rights reserved.
KeywordMagnetic Materials Biomaterials Nanoparticles Mycoplasma Pneumonia
SubtypeArticle
WOS HeadingsScience & Technology ; Technology ; Physical Sciences
DOI10.1016/j.matlet.2014.08.135
URL查看原文
Indexed BySCI
Language英语
WOS KeywordPEROXIDASE-LIKE ACTIVITY ; C-REACTIVE PROTEIN ; MAGNETIC NANOPARTICLES ; HRP ; PARTICLES
WOS Research AreaMaterials Science ; Physics
WOS SubjectMaterials Science, Multidisciplinary ; Physics, Applied
WOS IDWOS:000345469700031
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/11870
Collection研究所(批量导入)
Affiliation1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China
2.China Acad Inspect & Quarantine, Beijing 100123, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Yang, Mingzhu,Guan, Yueping,Yang, Yu,et al. A sensitive and rapid immunoassay for mycoplasma pneumonia based on Fe3O4 nanoparticles[J]. MATERIALS LETTERS,2014,137(DEC.):113-116.
APA Yang, Mingzhu,Guan, Yueping,Yang, Yu,Xia, Tingting,Xiong, Wubin,&Guo, Chen.(2014).A sensitive and rapid immunoassay for mycoplasma pneumonia based on Fe3O4 nanoparticles.MATERIALS LETTERS,137(DEC.),113-116.
MLA Yang, Mingzhu,et al."A sensitive and rapid immunoassay for mycoplasma pneumonia based on Fe3O4 nanoparticles".MATERIALS LETTERS 137.DEC.(2014):113-116.
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