Knowledge Management System Of Institute of process engineering,CAS
| Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants | |
| Zhao, Y.1,3; Stiles, A. R.1; Saxena, P. K.2; Liu, C. Z.1 | |
| 2013-03-01 | |
| Source Publication | BIOLOGIA PLANTARUM
 |
| ISSN | 0006-3134 |
| Volume | 57Issue:1Pages:189-192 |
| Abstract | An efficient in vitro plant regeneration system has been developed using dark preincubated leaf explants of Rhodiola crenulata, a traditional Tibetan medicinal plant. The leaf explants, preincubated in the dark for 5 d, developed an average of 9.1 shoots per explant on a medium containing 15 mu M N (6)-benzyladenine (BA) and 2.5 mu M gibberellic acid (GA(3)). The biochemical mechanism underlying dark-induced shoot organogenesis was investigated by measuring polyphenol oxidase (PPO) activity. Dark preincubation significantly reduced PPO activity in leaf explants during the initial period of shoot organogenesis and reduced browning compared to explants cultured in the light. Up to 88.4 % of the regenerated shoots formed roots and developed into complete plantlets on a medium containing 5 mu M indoleacetic acid (IAA) within 25 d. Approximately 82 % of the regenerated plantlets survived transplantation and grew vigorously in the greenhouse. |
| Keyword | Auxin Cytokinin Gibberellic Acid Polyphenol Oxidase |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s10535-012-0261-5 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | IN-VITRO ; SOMATIC EMBRYOGENESIS ; PLANT-REGENERATION ; TISSUE-CULTURES ; GROWTH |
| WOS Research Area | Plant Sciences |
| WOS Subject | Plant Sciences |
| WOS ID | WOS:000318647800029 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/13316 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 2.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Zhao, Y.,Stiles, A. R.,Saxena, P. K.,et al. Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants[J]. BIOLOGIA PLANTARUM,2013,57(1):189-192. |
| APA | Zhao, Y.,Stiles, A. R.,Saxena, P. K.,&Liu, C. Z..(2013).Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants.BIOLOGIA PLANTARUM,57(1),189-192. |
| MLA | Zhao, Y.,et al."Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants".BIOLOGIA PLANTARUM 57.1(2013):189-192. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Dark preincubation i（960KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | Application Full Text | |
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