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Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants
Zhao, Y.1,3; Stiles, A. R.1; Saxena, P. K.2; Liu, C. Z.1
2013-03-01
Source PublicationBIOLOGIA PLANTARUM
ISSN0006-3134
Volume57Issue:1Pages:189-192
AbstractAn efficient in vitro plant regeneration system has been developed using dark preincubated leaf explants of Rhodiola crenulata, a traditional Tibetan medicinal plant. The leaf explants, preincubated in the dark for 5 d, developed an average of 9.1 shoots per explant on a medium containing 15 mu M N (6)-benzyladenine (BA) and 2.5 mu M gibberellic acid (GA(3)). The biochemical mechanism underlying dark-induced shoot organogenesis was investigated by measuring polyphenol oxidase (PPO) activity. Dark preincubation significantly reduced PPO activity in leaf explants during the initial period of shoot organogenesis and reduced browning compared to explants cultured in the light. Up to 88.4 % of the regenerated shoots formed roots and developed into complete plantlets on a medium containing 5 mu M indoleacetic acid (IAA) within 25 d. Approximately 82 % of the regenerated plantlets survived transplantation and grew vigorously in the greenhouse.
KeywordAuxin Cytokinin Gibberellic Acid Polyphenol Oxidase
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10535-012-0261-5
Indexed BySCI
Language英语
WOS KeywordIN-VITRO ; SOMATIC EMBRYOGENESIS ; PLANT-REGENERATION ; TISSUE-CULTURES ; GROWTH
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000318647800029
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13316
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
2.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Y.,Stiles, A. R.,Saxena, P. K.,et al. Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants[J]. BIOLOGIA PLANTARUM,2013,57(1):189-192.
APA Zhao, Y.,Stiles, A. R.,Saxena, P. K.,&Liu, C. Z..(2013).Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants.BIOLOGIA PLANTARUM,57(1),189-192.
MLA Zhao, Y.,et al."Dark preincubation improves shoot organogenesis from Rhodiola crenulata leaf explants".BIOLOGIA PLANTARUM 57.1(2013):189-192.
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