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Comparative characterization of proteins secreted by Neurospora sitophila in solid-state and submerged fermentation
Li, Yanjun1,2; Peng, Xiaowei1; Chen, Hongzhang1
2013-10-01
Source PublicationJOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN1389-1723
Volume116Issue:4Pages:493-498
AbstractAlthough submerged fermentation (SmF) accounts for most of current enzyme industries, it has been reported that solid-state fermentation (SSF) can produce higher enzyme yields in laboratory scale. In order to understand the reasons contributing to high enzyme production in SSF, this study compared the cellulase activities and secretomes of Neurospora sitophila cultured in SSF and SmF using steam exploded wheat straw as carbon source and enzyme inducer. The total amounts of protein and biomass (glucosamine content) in SSF were respectively 30 and 2.8 times of those in SmF. The CMCase, FPA and beta-glucoside activities in SSF were 53-181 times of those in SmF. Both in SSF and SmF, N. sitophila secreted the most critical cellulases and hemicellulases known for Trichoderma reesei, although a beta-xylosidase was exclusively identified in SSF. Six endoglucanases were identified in N. sitophila secretion with the high CMCase activity. The non-enzyme proteins in SSF were involved in fungal mycelia growth and conidiation; while those in SmF were more related to glycometabolism and stress tolerance. This revealed that SSF more likely serves as a natural habitat for filamentous fungi to facilitate the enzyme secretion. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.
KeywordLignocellulose Fungal Secretome Cellulase Activity Solid-state Fermentation Submerged Fermentation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jbiosc.2013.04.001
Indexed BySCI
Language英语
WOS KeywordTRICHODERMA-REESEI ; FILAMENTOUS FUNGUS ; TRANSCRIPTIONAL REGULATION ; EXTRACELLULAR PROTEINS ; CELLULASE PRODUCTION ; ENZYME-PRODUCTION ; CARBON SOURCE ; CRASSA ; HEMICELLULASES ; CONIDIATION
WOS Research AreaBiotechnology & Applied Microbiology ; Food Science & Technology
WOS SubjectBiotechnology & Applied Microbiology ; Food Science & Technology
WOS IDWOS:000326559500014
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13318
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Li, Yanjun,Peng, Xiaowei,Chen, Hongzhang. Comparative characterization of proteins secreted by Neurospora sitophila in solid-state and submerged fermentation[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2013,116(4):493-498.
APA Li, Yanjun,Peng, Xiaowei,&Chen, Hongzhang.(2013).Comparative characterization of proteins secreted by Neurospora sitophila in solid-state and submerged fermentation.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,116(4),493-498.
MLA Li, Yanjun,et al."Comparative characterization of proteins secreted by Neurospora sitophila in solid-state and submerged fermentation".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 116.4(2013):493-498.
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