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Magnetic field intensified bi-enzyme system with in situ cofactor regeneration supported by magnetic nanoparticles
Zheng, Muqing1; Su, Zhiguo1; Ji, Xiaoyuan1; Ma, Guanghui1; Wang, Ping2,3; Zhang, Songping1
2013-10-20
Source PublicationJOURNAL OF BIOTECHNOLOGY
ISSN0168-1656
Volume168Issue:2Pages:212-217
AbstractEfficient dynamic interactions among cofactor, enzymes and substrate molecules are of primary importance for multi-step enzymatic reactions with in situ cofactor regeneration. Here we showed for the first time that the above dynamic interactions could be significantly intensified by exerting an external alternating magnetic field on magnetic nanoparticles-supported multi-enzymatic system so that the inter-particle collisions due to Brownian motion of nanoparticles could be improved. To that end, a multienzyme system including glutamate dehydrogenase (GluDH), glucose dehydrogenase (GDH) and cofactor NAD(H) were separately immobilized on silica coated Fe3O4 magnetic nanoparticles with an average diameter of 105 nm, and the effect of magnetic field strength and frequency on the kinetics of the coupled bi-enzyme reaction was investigated. It was found that at low magnetic field frequency (25 Hz and 100 Hz), increasing magnetic field strength from 9.8 to 161.1 Gs led to only very slight increase in reaction rate of the coupled bi-enzyme reaction expressed by glucose consumption rate. At higher magnetic field of 200 Hz and 500 Hz, reaction rate increased significantly with increase of magnetic field strength. When the magnetic field frequency was kept at 500 Hz, the reaction rate increased from 3.89 mu M/min to 8.11 mu M/min by increasing magnetic field strength from 1.3 to 14.2 Gs. The immobilized bi-enzyme system also showed good reusability and stability in the magnetic field (500 Hz, 14.2 Gs), that about 46% of original activity could be retained after 33 repeated uses, accounting for totally 34 days continuous operation. These results demonstrated the feasibility in intensifying molecular interactions among magnetic nanoparticle-supported multienzymes by using nano-magnetic stirrer for efficient multi-step transformations. (C) 2013 Elsevier B. V. All rights reserved.
KeywordMultienzyme Magnetic Nanoparticles Cofactor Regeneration Magnetic Field Immobilization
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.jbiotec.2013.05.016
Indexed BySCI
Language英语
WOS KeywordCOLLOIDAL SILICA COATINGS ; MOBILITY ; ENZYMES ; PARTICLES ; AGENT ; DNA
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000325978300013
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13343
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA
3.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA
Recommended Citation
GB/T 7714
Zheng, Muqing,Su, Zhiguo,Ji, Xiaoyuan,et al. Magnetic field intensified bi-enzyme system with in situ cofactor regeneration supported by magnetic nanoparticles[J]. JOURNAL OF BIOTECHNOLOGY,2013,168(2):212-217.
APA Zheng, Muqing,Su, Zhiguo,Ji, Xiaoyuan,Ma, Guanghui,Wang, Ping,&Zhang, Songping.(2013).Magnetic field intensified bi-enzyme system with in situ cofactor regeneration supported by magnetic nanoparticles.JOURNAL OF BIOTECHNOLOGY,168(2),212-217.
MLA Zheng, Muqing,et al."Magnetic field intensified bi-enzyme system with in situ cofactor regeneration supported by magnetic nanoparticles".JOURNAL OF BIOTECHNOLOGY 168.2(2013):212-217.
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