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Enzymatic Hydrolysis and Succinic Acid Fermentation from Steam-Exploded Corn Stalk at High Solid Concentration by Recombinant Escherichia coli
Wu, Dexi1; Li, Qiang2; Wang, Dan3; Dong, Yugang1
2013-08-01
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume170Issue:8Pages:1942-1949
AbstractSteam-exploded corn stalk biomass was used as the substrate for succinic acid production via lignocellulose enzymatic hydrolysis and fermentation. Succinic acid fermentation was investigated in Escherichia coli strains overexpressing cyanobacterium Anabaena sp. 7120 ecaA gene encoding carbonic anhydrase (CA). For the washed steam-exploded corn stalk at 30 % substrate concentration, i.e., 30 % water-insoluble solids (WIS), enzymatic hydrolysis yielded 97.5 g/l glucose solution and a cellulose conversion of 73.6 %, thus a high succinic acid level up to 38.6 g/l. With the unwashed steam-exploded corn stalk, though a cellulose conversion of 71.2 % was obtained in hydrolysis at 30 % solid concentration (27.9 % WIS), its hydrolysate did not ferment at all, and the hydrolysate of 25 % solid loading containing 3.8 g/l acetic acid and 168.2 mg/l furfural exerted a strong inhibition on succinic acid production.
KeywordSuccinic Acid Escherichia Coli High Solid Concentration Enzymatic Hydrolysis Fermentation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS KeywordSIMULTANEOUS SACCHARIFICATION ; BIOREFINING STRATEGY ; ETHANOL ; GLUCOSE ; INHIBITION ; BIOMASS
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000322619100012
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13358
Collection研究所(批量导入)
Affiliation1.Sun Yat Sen Univ, Affiliated Hosp 1, Dept Cardiol, Guangzhou 510080, Guangdong, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Chongqing Univ, Coll Chem & Chem Engn, Chongqing 400044, Peoples R China
Recommended Citation
GB/T 7714
Wu, Dexi,Li, Qiang,Wang, Dan,et al. Enzymatic Hydrolysis and Succinic Acid Fermentation from Steam-Exploded Corn Stalk at High Solid Concentration by Recombinant Escherichia coli[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2013,170(8):1942-1949.
APA Wu, Dexi,Li, Qiang,Wang, Dan,&Dong, Yugang.(2013).Enzymatic Hydrolysis and Succinic Acid Fermentation from Steam-Exploded Corn Stalk at High Solid Concentration by Recombinant Escherichia coli.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,170(8),1942-1949.
MLA Wu, Dexi,et al."Enzymatic Hydrolysis and Succinic Acid Fermentation from Steam-Exploded Corn Stalk at High Solid Concentration by Recombinant Escherichia coli".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 170.8(2013):1942-1949.
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