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Studies on the co-immobilized GOD/CAT on cross-linked chitosan microsphere modified by lysine
Zhang, Jie1; Zhou, Xiaohua1; Wang, Dan1; Wang, Yingli1; Zhou, Xing2; Wang, Honghui1; Li, Qiang3; Tan, Shiyu1
2013-12-15
Source PublicationJOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume97Issue:0Pages:80-86
AbstractGlucose oxidase (GOD) and catalase (CAT) were co-immobilized on a novel crosslinked chitosan resin modified by L-lysine. The immobilized system exhibited the best performance when the immobilization process was conducted at 4 degrees C for 3 h with GOD/CAT activity ratio of 1:18 and pH 7.0. Under the optimum pH 4.0 and temperature 40 degrees C, the enzyme system could achieve to the maximum conversion of glucose, which was 68.9%. Acidoresistance and thermotolerance of the enzymes were both strengthened. The Km of the co-immobilized enzyme system obtained in this study was 31.02 mM. The GOD enzyme immobilized in this system could maintain 94% of initial activity after the 12th operational cycle. 95.8% of initial activity was left after 50 weeks' storage at 4 degrees C. GOD achieved better performance in this new immobilized enzyme system due to its effective elimination of H2O2, which showed potential applications for various commercial uses. (C) 2013 Elsevier B.V. All rights reserved.
KeywordCrosslinked Chitosan Microspheres Co-immobilized God/cat God Performance H2o2 Lysine
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Language英语
WOS KeywordGLUCOSE-OXIDASE ; COVALENT IMMOBILIZATION ; ENZYME IMMOBILIZATION ; CALCIUM GLUCONATE ; MICROBIAL LIPASE ; CATALASE ; MEMBRANES ; OXIDATION ; KINETICS ; LINKING
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Physical
WOS IDWOS:000327005000013
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13361
Collection研究所(批量导入)
Affiliation1.Chongqing Univ, Coll Chem & Chem Engn, Chongqing 400044, Peoples R China
2.Chongqing Acad Chinese Mat Med, TCM Pharmacochem Inst, Chongqing 400065, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Jie,Zhou, Xiaohua,Wang, Dan,et al. Studies on the co-immobilized GOD/CAT on cross-linked chitosan microsphere modified by lysine[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2013,97(0):80-86.
APA Zhang, Jie.,Zhou, Xiaohua.,Wang, Dan.,Wang, Yingli.,Zhou, Xing.,...&Tan, Shiyu.(2013).Studies on the co-immobilized GOD/CAT on cross-linked chitosan microsphere modified by lysine.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,97(0),80-86.
MLA Zhang, Jie,et al."Studies on the co-immobilized GOD/CAT on cross-linked chitosan microsphere modified by lysine".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 97.0(2013):80-86.
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