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Immobilization of Cross-Linked Phenylalanine Ammonia Lyase Aggregates in Microporous Silica Gel
Cui, Jian Dong1,2,3; Li, Lian Lian1; Bian, Hong Jie
2013-11-15
Source PublicationPLOS ONE
ISSN1932-6203
Volume8Issue:11Pages:80581
AbstractA separable and highly-stable enzyme system was developed by adsorption of phenylalanine ammonia lyase (PAL) from Rhodotorula glutinis in amino-functionalized macroporous silica gel and subsequent enzyme crosslinking. This resulted in the formation of cross-linked enzyme aggregates (PAL-CLEAs) into macroporous silica gel (MSG-CLEAs). The effect of adsorptive conditions, type of aggregating agent, its concentration as well as that of cross-linking agent was studied. MSG-CLEAs production was most effective using ammonium sulfate (40%-saturation), followed by cross-linking for 1 h with 1.5% (v/v) glutaraldehyde. The resulting MSG-CLEAs extended the optimal temperature and pH range compared to free PAL and PAL-CLEAs. Moreover, MSG-CLEAs exhibited the excellent stability of the enzyme against various deactivating conditions such as temperature and denaturants, and showed higher storage stability compared to the free PAL and the conventional PAL-CLEAs. Such as, after 6 h incubation at 60 degrees C, the MSG-CLEAs still retained more than 47% of the initial activity whereas PAL-CLEAs only retained 7% of the initial activity. Especially, the MSG-CLEAs exhibited good reusability due to its suitable size and active properties. These results indicated that PAL-CLEAs on MSG might be used as a feasible and efficient solution for improving properties of immobilized enzyme in industrial application.
SubtypeArticle
WOS HeadingsScience & Technology
DOI10.1371/journal.pone.0080581
Indexed BySCI
Language英语
WOS KeywordENZYME STABILIZATION ; CLEAS ; BIOCATALYSTS ; EFFICIENT ; ACYLASE ; LIPASES
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000327258600071
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13366
Collection研究所(批量导入)
Affiliation1.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Res Ctr Fermentat Engn Hebei, Shijiazhuang, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
3.Tianjin Univ Sci & Technol, Minist Educ, Key Lab Ind Microbiol, Tai Da Dev Area, Tianjin, Peoples R China
Recommended Citation
GB/T 7714
Cui, Jian Dong,Li, Lian Lian,Bian, Hong Jie. Immobilization of Cross-Linked Phenylalanine Ammonia Lyase Aggregates in Microporous Silica Gel[J]. PLOS ONE,2013,8(11):80581.
APA Cui, Jian Dong,Li, Lian Lian,&Bian, Hong Jie.(2013).Immobilization of Cross-Linked Phenylalanine Ammonia Lyase Aggregates in Microporous Silica Gel.PLOS ONE,8(11),80581.
MLA Cui, Jian Dong,et al."Immobilization of Cross-Linked Phenylalanine Ammonia Lyase Aggregates in Microporous Silica Gel".PLOS ONE 8.11(2013):80581.
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