Knowledge Management System Of Institute of process engineering,CAS
| Immobilization of Cross-Linked Phenylalanine Ammonia Lyase Aggregates in Microporous Silica Gel | |
| Cui, Jian Dong1,2,3; Li, Lian Lian1; Bian, Hong Jie | |
| 2013-11-15 | |
| Source Publication | PLOS ONE
 |
| ISSN | 1932-6203 |
| Volume | 8Issue:11Pages:80581 |
| Abstract | A separable and highly-stable enzyme system was developed by adsorption of phenylalanine ammonia lyase (PAL) from Rhodotorula glutinis in amino-functionalized macroporous silica gel and subsequent enzyme crosslinking. This resulted in the formation of cross-linked enzyme aggregates (PAL-CLEAs) into macroporous silica gel (MSG-CLEAs). The effect of adsorptive conditions, type of aggregating agent, its concentration as well as that of cross-linking agent was studied. MSG-CLEAs production was most effective using ammonium sulfate (40%-saturation), followed by cross-linking for 1 h with 1.5% (v/v) glutaraldehyde. The resulting MSG-CLEAs extended the optimal temperature and pH range compared to free PAL and PAL-CLEAs. Moreover, MSG-CLEAs exhibited the excellent stability of the enzyme against various deactivating conditions such as temperature and denaturants, and showed higher storage stability compared to the free PAL and the conventional PAL-CLEAs. Such as, after 6 h incubation at 60 degrees C, the MSG-CLEAs still retained more than 47% of the initial activity whereas PAL-CLEAs only retained 7% of the initial activity. Especially, the MSG-CLEAs exhibited good reusability due to its suitable size and active properties. These results indicated that PAL-CLEAs on MSG might be used as a feasible and efficient solution for improving properties of immobilized enzyme in industrial application. |
| Subtype | Article |
| WOS Headings | Science & Technology |
| DOI | 10.1371/journal.pone.0080581 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | ENZYME STABILIZATION ; CLEAS ; BIOCATALYSTS ; EFFICIENT ; ACYLASE ; LIPASES |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000327258600071 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/13366 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Res Ctr Fermentat Engn Hebei, Shijiazhuang, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 3.Tianjin Univ Sci & Technol, Minist Educ, Key Lab Ind Microbiol, Tai Da Dev Area, Tianjin, Peoples R China |
| Recommended Citation GB/T 7714 | Cui, Jian Dong,Li, Lian Lian,Bian, Hong Jie. Immobilization of Cross-Linked Phenylalanine Ammonia Lyase Aggregates in Microporous Silica Gel[J]. PLOS ONE,2013,8(11):80581. |
| APA | Cui, Jian Dong,Li, Lian Lian,&Bian, Hong Jie.(2013).Immobilization of Cross-Linked Phenylalanine Ammonia Lyase Aggregates in Microporous Silica Gel.PLOS ONE,8(11),80581. |
| MLA | Cui, Jian Dong,et al."Immobilization of Cross-Linked Phenylalanine Ammonia Lyase Aggregates in Microporous Silica Gel".PLOS ONE 8.11(2013):80581. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Immobilization of Cr（1211KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | Application Full Text | |
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