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Immobilization of Papain in Biosilica Matrix and Its Catalytic Property
Zhou Liya1; Wang Cui1; Jiang Yanjun1,2; Gao Jing1
2013-06-01
Source PublicationCHINESE JOURNAL OF CHEMICAL ENGINEERING
ISSN1004-9541
Volume21Issue:6Pages:670-675
AbstractA novel method was developed for papain immobilization through a biomimetic silicification process induced by papain. By incubating papain in a silica precursor solution, the papain-silica composite formed rapidly and papain was encapsulated. The encapsulation efficiency and the recovery activity were 82.60% and 83.09%, respectively. Compared with enzymes and biomolecules immobilized in biosilica matrix in the presence of additional silica-precipitating species, this papain encapsulation process, a biomimetic approach, realized high encapsulation efficiency by its autosilification activity under mild conditions (near-neutral pH and ambient temperature). Furthermore, the encapsulated papain exhibits enhanced thermal, pH, recycling and storage stabilities. Kinetic analysis showed that the biomimetic silica matrix did not significantly hinder the mass transport of substrate or the release of product.
KeywordPapain Silica Matrix Encapsulation Biosilicification Biomimetic
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1016/S1004-9541(13)60528-5
Indexed BySCI
Language英语
WOS KeywordBIOMIMETICALLY SYNTHESIZED SILICA ; ENZYME ENCAPSULATION ; PROTEIN ; EFFICIENT ; ENTRAPMENT ; LYSOZYME ; SUPPORT ; TITANIA
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
WOS IDWOS:000322510200011
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13374
Collection研究所(批量导入)
Affiliation1.Hebei Univ Technol, Sch Chem Engn & Technol, Tianjin 300130, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhou Liya,Wang Cui,Jiang Yanjun,et al. Immobilization of Papain in Biosilica Matrix and Its Catalytic Property[J]. CHINESE JOURNAL OF CHEMICAL ENGINEERING,2013,21(6):670-675.
APA Zhou Liya,Wang Cui,Jiang Yanjun,&Gao Jing.(2013).Immobilization of Papain in Biosilica Matrix and Its Catalytic Property.CHINESE JOURNAL OF CHEMICAL ENGINEERING,21(6),670-675.
MLA Zhou Liya,et al."Immobilization of Papain in Biosilica Matrix and Its Catalytic Property".CHINESE JOURNAL OF CHEMICAL ENGINEERING 21.6(2013):670-675.
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仿生硅化固定木瓜蛋白酶及其催化性能研究_(784KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
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