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Analysis of Mogroside V in Siraitia grosvenorii with Micelle-mediated Cloud-Point Extraction
Deng, Fuli1,2,3; Liang, Xiangfeng1; Yang, Liangrong1; Liu, Qingfen1; Liu, Huizhou1,2
2013-07-01
Source PublicationPHYTOCHEMICAL ANALYSIS
Volume24Issue:4Pages:381-385
AbstractIntroduction Mogroside V is the main effective ingredient of Siraitia grosvenorii used as a natural sweet food as well as a traditional Chinese medicine. The sample pre-treatment prior to chromatographic analysis requires large amounts of toxic organic solvents and is time consuming. Objective To develop an effective and simple method for extracting and determining mogroside V of Siraitia grosvenorii. Methods Mogroside V was extracted and preconcentrated by micelle-mediated cloud-point extraction with nonionic surfactant isotridecyl poly (ethylene glycol) ether (Genapol (R) X-080). The obtained solutions containing mogroside V were analysed by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. The chromatographic separation was performed on a C18-column using gradient elution with acetonitrile and water at 203nm. Results The cloud-point extraction yield was 80.7% while the pre-concentration factor was about 10.8. The limit of detection was 0.75 mu g/mL and the limit of quantification was 2 mu g/mL. The relative standard deviations for intra- and interday precisions of mogroside V were less than 8.68% and 5.78%, respectively, and the recoveries were between 85.1% and 103.6%. Conclusion The HPLC-UV method based on micelle-mediated cloud-point extraction for determination mogroside V in Siraitia grosvenorii was environmentally friendly, simple and sensitive. Copyright (c) 2013 John Wiley & Sons, Ltd.
KeywordCloud-point Extraction Hplc Genapol (r) X-080 Mogroside v Siraitia Grosvenorii
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Language英语
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; POLYCYCLIC AROMATIC-HYDROCARBONS ; CUCURBITANE GLYCOSIDES ; PRECONCENTRATION ; SEPARATION ; MEDICINE ; SYSTEMS ; FRUITS ; SERUM
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences ; Chemistry
WOS SubjectBiochemical Research Methods ; Plant Sciences ; Chemistry, Analytical
WOS IDWOS:000320558200013
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13390
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Deng, Fuli,Liang, Xiangfeng,Yang, Liangrong,et al. Analysis of Mogroside V in Siraitia grosvenorii with Micelle-mediated Cloud-Point Extraction[J]. PHYTOCHEMICAL ANALYSIS,2013,24(4):381-385.
APA Deng, Fuli,Liang, Xiangfeng,Yang, Liangrong,Liu, Qingfen,&Liu, Huizhou.(2013).Analysis of Mogroside V in Siraitia grosvenorii with Micelle-mediated Cloud-Point Extraction.PHYTOCHEMICAL ANALYSIS,24(4),381-385.
MLA Deng, Fuli,et al."Analysis of Mogroside V in Siraitia grosvenorii with Micelle-mediated Cloud-Point Extraction".PHYTOCHEMICAL ANALYSIS 24.4(2013):381-385.
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