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Ultrasound-intensified laccase production from Trametes versicolor
Wang, Feng1; Ma, An-Zhou2; Guo, Chen1; Zhuang, Guo-Qiang2; Liu, Chun-Zhao1
2013
Source PublicationULTRASONICS SONOCHEMISTRY
ISSN1350-4177
Volume20Issue:1Pages:118-124
AbstractAn efficient intermittent ultrasonic treatment strategy was developed to improve laccase production from Trametes versicolor mycelia cultures. The optimized strategy consisted of exposing 2-day-old mycelia cultures to 5-min ultrasonic treatments for two times with a 12-h interval at the fixed ultrasonic power and frequency (120W, 40 kHz). After 5 days of culture, this strategy produced the highest extracellular laccase activity of 588.9 U/L among all treatments tested which was 1.8-fold greater than the control without ultrasound treatment. The ultrasonic treatment resulted in a higher pellet porosity that facilitated the mass transfer of nutrients and metabolites from the pellets to the surrounding liquid. Furthermore, the ultrasonic treatment induced the expression of the laccase gene (lcc), which correlated with a sharp increase in both extracellular and intracellular laccase activity. This is the first study to find positive effects of ultrasound on gene expression in fungal cells. These results provide a basis for understanding the stimulation of metabolite production and process intensification by ultrasonic treatment in filamentous fungal culture. (C) 2012 Elsevier B.V. All rights reserved.
KeywordUltrasound Trametes Versicolor Laccase Mrna
SubtypeArticle
WOS HeadingsScience & Technology ; Technology ; Physical Sciences
DOI10.1016/j.ultsonch.2012.05.003
Indexed BySCI
Language英语
WOS KeywordHETEROLOGOUS PROTEIN-PRODUCTION ; ASPERGILLUS-NIGER FERMENTATION ; KINASE PATHWAYS ; MEMBRANE ; CULTURE ; GROWTH ; FUNGI ; PURIFICATION ; MORPHOLOGY ; STABILITY
WOS Research AreaAcoustics ; Chemistry
WOS SubjectAcoustics ; Chemistry, Multidisciplinary
WOS IDWOS:000311130000018
Citation statistics
Cited Times:38[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13410
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China
Recommended Citation
GB/T 7714
Wang, Feng,Ma, An-Zhou,Guo, Chen,et al. Ultrasound-intensified laccase production from Trametes versicolor[J]. ULTRASONICS SONOCHEMISTRY,2013,20(1):118-124.
APA Wang, Feng,Ma, An-Zhou,Guo, Chen,Zhuang, Guo-Qiang,&Liu, Chun-Zhao.(2013).Ultrasound-intensified laccase production from Trametes versicolor.ULTRASONICS SONOCHEMISTRY,20(1),118-124.
MLA Wang, Feng,et al."Ultrasound-intensified laccase production from Trametes versicolor".ULTRASONICS SONOCHEMISTRY 20.1(2013):118-124.
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