CAS OpenIR  > 研究所(批量导入)
A Novel Bifunctional Protein TNFR2-Fc-IL-1ra (TFI): Expression, Purification and its Neutralization Activity of Inflammatory Factors
Xie, Bo1; Liu, Shan1; Wu, Shuyin2; Chang, Alan3; Jin, Weiyuan2; Guo, Zhixiang2; Ye, Shuaidong2; Hui, Mizhou1,2
2013-06-01
Source PublicationMOLECULAR BIOTECHNOLOGY
Volume54Issue:2Pages:141-147
AbstractTumor necrosis factor receptor (TNF) and internleukin-1 (IL-1) are the most potent proinflammatory cytokines involving in autoimmune and inflammatory human diseases. Many anti-inflammatory agents have been exploited for anti-inflammation treatments by targeting cytokines including TNF and IL-1. Theoretically, simultaneously neutralizing or blocking two important inflammatory mediators may achieve a synergistic therapeutic effect. We have developed a recombinant fusion protein, TNFR2-Fc-IL-1ra (TFI), which consists of a TNF-neutralizing domain that specifically binds to TNF-alpha, an IL-1 receptor antagonist domain, and a dimerization Fc portion of human IgG1, for bifunctional inflammatory inhibitor. Recombinant DNA expressing the sequence of this fusion protein was expressed in CHO-S cells. The protein product was purified using a two-step purification protocol and the identity of the protein was confirmed by western blot analysis. The purified recombinant protein had a purity of about 98 % as determined by HPLC, and a molecular mass of 164.6 kDa as determined by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. The results of cell binding inhibition indicate that TFI was able to strongly neutralize TNF activity and antagonize IL-1r activity, suggesting that TFI may be used as a bifunctional ligand with enhanced anti-inflammatory effect. The result obtained in this study may provide a platform for extending bifunctional anti-inflammatory drug development.
KeywordBifunctional Protein Gc-rich Vector Large Scale Cultivation Bioreactor Affinity Purification
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS KeywordINTERLEUKIN-1 RECEPTOR ANTAGONIST ; RHEUMATOID-ARTHRITIS ; THERAPEUTIC TARGETS ; DISEASES ; MECHANISMS ; CYTOKINES ; ALPHA ; DNA
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000318308400003
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13458
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.AmProt China Inc, Hangzhou 310019, Zhejiang, Peoples R China
3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China
Recommended Citation
GB/T 7714
Xie, Bo,Liu, Shan,Wu, Shuyin,et al. A Novel Bifunctional Protein TNFR2-Fc-IL-1ra (TFI): Expression, Purification and its Neutralization Activity of Inflammatory Factors[J]. MOLECULAR BIOTECHNOLOGY,2013,54(2):141-147.
APA Xie, Bo.,Liu, Shan.,Wu, Shuyin.,Chang, Alan.,Jin, Weiyuan.,...&Hui, Mizhou.(2013).A Novel Bifunctional Protein TNFR2-Fc-IL-1ra (TFI): Expression, Purification and its Neutralization Activity of Inflammatory Factors.MOLECULAR BIOTECHNOLOGY,54(2),141-147.
MLA Xie, Bo,et al."A Novel Bifunctional Protein TNFR2-Fc-IL-1ra (TFI): Expression, Purification and its Neutralization Activity of Inflammatory Factors".MOLECULAR BIOTECHNOLOGY 54.2(2013):141-147.
Files in This Item:
File Name/Size DocType Version Access License
A Novel Bifunctional(457KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xie, Bo]'s Articles
[Liu, Shan]'s Articles
[Wu, Shuyin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xie, Bo]'s Articles
[Liu, Shan]'s Articles
[Wu, Shuyin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xie, Bo]'s Articles
[Liu, Shan]'s Articles
[Wu, Shuyin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.