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Comparison of fully-porous beads and cored beads in size exclusion chromatography for protein purification
Luo, Jian1; Zhou, Weiqing1; Su, Zhiguo1; Ma, Guanghui1; Gu, Tingyue2
2013-10-11
Source PublicationCHEMICAL ENGINEERING SCIENCE
Volume102Issue:0Pages:99-105
AbstractSize-exclusion chromatography (SEC) relies exclusively on intraparticle diffusion to separate solutes of different molecular sizes and shapes. Thus, its feed volume can only be a small fraction of the column volume. Much larger columns are required for SEC than other forms of liquid chromatography. Becasue of this, SEC often employs less expensive soft gels in large-scale applications to reduce costs. Excessive bed compression forces engineers to use pancake-shaped columns instead of more desirable slim columns during scale-up. Cored beads have impenetrable rigid cores that result in lower pressure drops and better pressure resistance. They also provide sharper peaks due to shortened radial distance for diffusion. Using a new general rate model for SEC with cored beads, this work demonstrated that cored beads performed better than fully-porous beads for myoglobin and ovalbumin separation through computer simulation. This theoretical work could encourge the research and product development of cored beads for large-scale SEC that has not been reported. (C) 2013 Elsevier Ltd. All rights reserved.
KeywordSize Exclusion Chromatography Cored Beads Modeling Protein Purification Mass Transfer
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
Indexed BySCI
Language英语
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; HEPATITIS-B-VIRUS ; PACKED-BEDS ; EXCHANGE CHROMATOGRAPHY ; PACKING MATERIALS ; MASS TRANSFER ; MEDIA ; MODEL ; SCALE ; FLOW
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
WOS IDWOS:000325945500010
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13465
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Ohio Univ, Dept Chem & Biomol Engn, Athens, OH 45701 USA
Recommended Citation
GB/T 7714
Luo, Jian,Zhou, Weiqing,Su, Zhiguo,et al. Comparison of fully-porous beads and cored beads in size exclusion chromatography for protein purification[J]. CHEMICAL ENGINEERING SCIENCE,2013,102(0):99-105.
APA Luo, Jian,Zhou, Weiqing,Su, Zhiguo,Ma, Guanghui,&Gu, Tingyue.(2013).Comparison of fully-porous beads and cored beads in size exclusion chromatography for protein purification.CHEMICAL ENGINEERING SCIENCE,102(0),99-105.
MLA Luo, Jian,et al."Comparison of fully-porous beads and cored beads in size exclusion chromatography for protein purification".CHEMICAL ENGINEERING SCIENCE 102.0(2013):99-105.
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