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A surfactant-coated lipase immobilized in magnetic nanoparticles for multicycle ethyl isovalerate enzymatic production
Mahmood, Iram1,3; Ahmad, Ishfaq2; Chen, Guo3; Liu Huizhou3
2013-04-15
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
Volume73Issue:0Pages:72-79
AbstractGum arabic coated magnetic Fe3O4 nanoparticles (GAMNP) were prepared by chemical co-precipitation method and their surface morphology, particle size and presence of polymer-coating was confirmed by various measurements, including transmission electron microscopy (TEM), X-ray diffraction (XRD), thermo gravimetric analysis (TGA), and Fourier transform infra red (FTIR) analysis. Magnetic particles were employed for their potential application as a support material for lipase immobilization. Glutaraldehyde was used as a coupling agent for efficient binding of lipase onto the magnetic carrier. For this purpose, the surface of a Candida rugosa lipase was initially coated with various surfactants, to stabilize enzyme in its open form, and then immobilized on to the support. This immobilized system was used as a biocatalyst for ethyl isovalerate, a flavor ester, production. The influence of various factors such as type of surfactant, optimum temperature and pH requirement, organic solvent used, amount of surfactant in coating lipase and effect of enzyme loadings on the esterification reaction were systematically studied. Different surfactants were used amongst which non-ionic surfactant performed better, showing about 80% esterification yield in 48 h as compared to cationic/anionic surfactants. Enhanced activity due to interfacial activation was observed for immobilized non-ionic surfactant-lipase complex. The immobilized surfactant coated lipase activity was retained after reusing seven times. (C) 2013 Elsevier B.V. All rights reserved.
KeywordMagnetic Nanoparticles Gum Arabic Lipase Esterification
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
Indexed BySCI
Language英语
WOS KeywordCANDIDA-RUGOSA LIPASE ; INTERFACIAL ACTIVATION ; ORGANIC-SOLVENTS ; FLAVOR ESTERS ; ENZYMES ; PARTICLES ; HYDROLYSIS ; CATALYSIS
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000317453400010
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13524
Collection研究所(批量导入)
Affiliation1.Natl Univ Sci & Technol, Sch Chem & Mat Engn, Islamabad 44000, Pakistan
2.Int Islamic Univ, Dept Math & Stat, Islamabad, Pakistan
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Mahmood, Iram,Ahmad, Ishfaq,Chen, Guo,et al. A surfactant-coated lipase immobilized in magnetic nanoparticles for multicycle ethyl isovalerate enzymatic production[J]. BIOCHEMICAL ENGINEERING JOURNAL,2013,73(0):72-79.
APA Mahmood, Iram,Ahmad, Ishfaq,Chen, Guo,&Liu Huizhou.(2013).A surfactant-coated lipase immobilized in magnetic nanoparticles for multicycle ethyl isovalerate enzymatic production.BIOCHEMICAL ENGINEERING JOURNAL,73(0),72-79.
MLA Mahmood, Iram,et al."A surfactant-coated lipase immobilized in magnetic nanoparticles for multicycle ethyl isovalerate enzymatic production".BIOCHEMICAL ENGINEERING JOURNAL 73.0(2013):72-79.
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