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Immobilization of beta-glucosidase onto Magnetic Nanoparticles and Evaluation of the Enzymatic Properties
Zhou, Ying1; Pan, Siyi1; Wei, Xuetuan2; Wang, Lufeng1; Liu, Yanlong1
2013
Source PublicationBIORESOURCES
Volume8Issue:2Pages:2605-2619
AbstractThis paper reports on a novel and efficient beta-glucosidase immobilization method using magnetic Fe3O4 nanoparticles as a carrier. Based on response surface methodology, the optimal immobilization conditions obtained were: glutaraldehyde (GA) concentration, 0.20%; enzyme concentration, 50.25 mu g/mL; cross-linking time, 2.21 h; and the maximum activity recovery reached 89.35%. The magnetic immobilized enzyme was characterized by Fourier transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), and vibrating sample magnetometer (VSM). FTIR revealed that beta-glucosidase was successfully immobilized on the magnetic nanoparticles. TEM showed that enzyme-magnetic nanoparticles possessed nano-scale size distribution. VSM confirmed that the enzyme-magnetic nanoparticles were superparamagnetic. The properties of the immobilized beta-glucosidase were improved, and the immobilized beta-glucosidase exhibited wider pH and temperature ranges of activation, higher accessibility of the substrate, better thermal stability, and better storage stability than that of the free enzyme. The enzyme-magnetic nanoparticles could be separated magnetically for easy reuse. Immobilization of beta-glucosidase onto the magnetic nanoparticles has the potential for industrial application.
KeywordMagnetic Nanoparticles Beta-glucosidase Immobilization Response Surface Methodology Characterization
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
Indexed BySCI
Language英语
WOS KeywordFE3O4 NANOPARTICLES ; ALPHA-AMYLASE ; BEADS ; CHITOSAN ; LIPASE
WOS Research AreaMaterials Science
WOS SubjectMaterials Science, Paper & Wood
WOS IDWOS:000320185500045
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13537
Collection研究所(批量导入)
Affiliation1.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Ying,Pan, Siyi,Wei, Xuetuan,et al. Immobilization of beta-glucosidase onto Magnetic Nanoparticles and Evaluation of the Enzymatic Properties[J]. BIORESOURCES,2013,8(2):2605-2619.
APA Zhou, Ying,Pan, Siyi,Wei, Xuetuan,Wang, Lufeng,&Liu, Yanlong.(2013).Immobilization of beta-glucosidase onto Magnetic Nanoparticles and Evaluation of the Enzymatic Properties.BIORESOURCES,8(2),2605-2619.
MLA Zhou, Ying,et al."Immobilization of beta-glucosidase onto Magnetic Nanoparticles and Evaluation of the Enzymatic Properties".BIORESOURCES 8.2(2013):2605-2619.
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