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Production of Ultra-high Molecular Weight Poly-gamma-Glutamic Acid with Bacillus licheniformis P-104 and Characterization of its Flocculation Properties
Zhao, Caifeng1; Zhang, Yewei1; Wei, Xuetuan2; Hu, Zhongbo1; Zhu, Feiyan1; Xu, Lin2; Luo, Mingfang3; Liu, Huizhou2
2013-06-01
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume170Issue:3Pages:562-572
AbstractA novel strain of Bacillus licheniformis P-104 was isolated from Chinese soybean paste to produce a bioflocculant. The bioflocculant was confirmed as ultra-high molecular weight poly-gamma-glutamic acid (gamma-PGA) using Fourier transform infrared spectrum, high-performance liquid chromatography, and gel permeation chromatography with multi-angle laser light scattering. The production technology and flocculation properties of gamma-PGA were investigated. By fed-batch fermentation in a 7-L bioreactor, the maximum gamma-PGA yield reached 41.6 g L-1 with a productivity rate of 1.07 g L-1 h(-1). The flocculating activity of gamma-PGA for kaolin suspension was 33.5 +/- 1.6 1/OD under the optimized flocculation conditions (6 mM Ca2+, 1.5 mg L-1 gamma-PGA, and pH 6.0). The optimized dosage of gamma-PGA for flocculation was just about 30 % of that of reported gamma-PGA produced by other strains. Moreover, the flocculation activity of gamma-PGA produced by strain P-104 was much higher than commercial gamma-PGA with the molecular weight ranging 200-500 kDa and 1,500-2,500 kDa. This study provided a promising strain and an efficient method for production of ultra-high molecular weight gamma-PGA which could be used as a potential green bioflocculant.
KeywordBacillus Licheniformis Fed-batch Fermentation Flocculation Gamma-polyglutamic Acid Ultra-high Molecular Weight
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS KeywordPOLY(GAMMA-GLUTAMIC ACID) ; POLYGLUTAMIC ACID ; BIOPOLYMER FLOCCULANT ; KAOLIN SUSPENSION ; SUBTILIS ; BIOFLOCCULANT ; CONFORMATION ; AERATION ; CULTURES ; ISOLATE
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000319134800008
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13538
Collection研究所(批量导入)
Affiliation1.Univ Chinese Acad Sci, Coll Mat Sci & Optoelect Technol, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Inst Microbiol, Technol Transfer Ctr, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Caifeng,Zhang, Yewei,Wei, Xuetuan,et al. Production of Ultra-high Molecular Weight Poly-gamma-Glutamic Acid with Bacillus licheniformis P-104 and Characterization of its Flocculation Properties[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2013,170(3):562-572.
APA Zhao, Caifeng.,Zhang, Yewei.,Wei, Xuetuan.,Hu, Zhongbo.,Zhu, Feiyan.,...&Liu, Huizhou.(2013).Production of Ultra-high Molecular Weight Poly-gamma-Glutamic Acid with Bacillus licheniformis P-104 and Characterization of its Flocculation Properties.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,170(3),562-572.
MLA Zhao, Caifeng,et al."Production of Ultra-high Molecular Weight Poly-gamma-Glutamic Acid with Bacillus licheniformis P-104 and Characterization of its Flocculation Properties".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 170.3(2013):562-572.
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