CAS OpenIR  > 研究所(批量导入)
Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing beta-poly (malic acid) production by Aureobasidium pullulans ipe-1
Cao, Weifeng1,2; Qi, Benkun1; Zhao, Juan3; Qiao, Changsheng4; Su, Yi1; Wan, Yinhua1
2013-05-01
Source PublicationJOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
Volume88Issue:5Pages:808-817
AbstractBACKGROUND: -poly(malic acid) (PMLA) can be used as a pro-drug or for a drug-delivery system. Effects of pH, dissolved oxygen concentration (DO) and stirring speed were investigated to improve PMLA production by A. pullulans ipe-1. RESULTS: The strain produced a high PMLA concentration when pH and DO remained at about 6.0 and above 70%, respectively, and the yeast-like cells were the main PMLA producers. To further promote PMLA production, the cultivation could be divided into three phases. In phase I, cell growth was accelerated by maintaining high DO (>70%) with a constant stirring speed of 800 rpm. In phase II, PMLA production was increased by controlling DO at 70% using the automatically controlled stirring speed. In phase III, PMLA production on per gram of glucose (Yp/s) was enhanced by keeping DO at 70%, and using a low stirring speed to decrease cell growth. Compared with batch cultures, a higher PMLA yield was obtained with this strategy, i.e. PMLA production and Yp/s increased by 15% and 18%, respectively. CONCLUSION: Control strategies for pH, DO and stirring speed provide a good reference for process development and optimization of PMLA production. (c) 2012 Society of Chemical Industry
Keyword-poly(Malic Acid) Aureobasidium Pullulans Dissolved Oxygen Concentration Stirring Speed
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology
Indexed BySCI
Language英语
WOS KeywordEXOPOLYSACCHARIDE PRODUCTION ; PHYSARUM-POLYCEPHALUM ; POLY(BETA-MALIC ACID) ; MOLECULAR-WEIGHT ; IMPELLER SPEED ; DRUG-DELIVERY ; CULTURE ; GROWTH ; FERMENTATION ; AGITATION
WOS Research AreaBiotechnology & Applied Microbiology ; Chemistry ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Chemical
WOS IDWOS:000317674400009
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13591
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Tianjin Univ Sci & Technol, Res Ctr Modern Anal Technol, Tianjin 300457, Peoples R China
4.Tianjin Univ Sci & Technol, Dept Bioengn, Tianjin 300457, Peoples R China
Recommended Citation
GB/T 7714
Cao, Weifeng,Qi, Benkun,Zhao, Juan,et al. Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing beta-poly (malic acid) production by Aureobasidium pullulans ipe-1[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2013,88(5):808-817.
APA Cao, Weifeng,Qi, Benkun,Zhao, Juan,Qiao, Changsheng,Su, Yi,&Wan, Yinhua.(2013).Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing beta-poly (malic acid) production by Aureobasidium pullulans ipe-1.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,88(5),808-817.
MLA Cao, Weifeng,et al."Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing beta-poly (malic acid) production by Aureobasidium pullulans ipe-1".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 88.5(2013):808-817.
Files in This Item:
File Name/Size DocType Version Access License
Control strategy of (230KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Cao, Weifeng]'s Articles
[Qi, Benkun]'s Articles
[Zhao, Juan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Cao, Weifeng]'s Articles
[Qi, Benkun]'s Articles
[Zhao, Juan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Cao, Weifeng]'s Articles
[Qi, Benkun]'s Articles
[Zhao, Juan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.