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Adsorption properties and preparative separation of phenylethanoid glycosides from Cistanche deserticola by use of macroporous resins
Liu, Boyan1,2; Ouyang, Jie2; Yuan, Xiaofan1; Wang, Liwei1; Zhao, Bing1
2013-10-15
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume937Issue:0Pages:84-90
AbstractA simple and efficient chromatographic method for large-scale preparative separation of phenylethanoid glycosides (mainly contain echinacoside and acteoside) from Cistanche deserticola was developed. The adsorption properties of eight macroporous resins were evaluated. Three selected resins were further screened depending on the adsorption kinetics curves, in which HPD300 resin showed the best separation efficiency. The adsorption isotherm data on HPD300 resin were fitted to the Freundlich equation in certain concentration range. The dynamic adsorption and desorption experiments were carried out on columns packed with HPD300 resin to optimize the separation process. The breakthrough curves showed that acteoside had a higher affinity to the resin than echinacoside. The contents of echinacoside and acteoside in the product increased from 1.79% and 1.43% in the crude extracts to 16.66% and 15.17%, with recovery yields of 80.41% and 90.17%, respectively. The purity of total phenylethanoid glycosides in the product was 76.58%. (c) 2013 Elsevier B.V. All rights reserved.
KeywordMacroporous Resin Phenylethanoid Glycosides Echinacoside Acteoside CistanC.e DesertiC.la Y. C. Ma Separation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Language英语
WOS KeywordCOUNTER-CURRENT CHROMATOGRAPHY ; DIODE-ARRAY DETECTION ; COMPETITIVE ADSORPTION ; MASS-SPECTROMETRY ; TUBULOSA ; CONSTITUENTS ; PURIFICATION ; OLIGOGLYCOSIDES ; NITROPHENOL ; FLAVONOIDS
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000325040400012
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13594
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Being Forestry Univ, Coll Biol Sci & Biotechnol, Dept Food Sci & Engn, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Liu, Boyan,Ouyang, Jie,Yuan, Xiaofan,et al. Adsorption properties and preparative separation of phenylethanoid glycosides from Cistanche deserticola by use of macroporous resins[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,937(0):84-90.
APA Liu, Boyan,Ouyang, Jie,Yuan, Xiaofan,Wang, Liwei,&Zhao, Bing.(2013).Adsorption properties and preparative separation of phenylethanoid glycosides from Cistanche deserticola by use of macroporous resins.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,937(0),84-90.
MLA Liu, Boyan,et al."Adsorption properties and preparative separation of phenylethanoid glycosides from Cistanche deserticola by use of macroporous resins".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 937.0(2013):84-90.
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