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Gas-Assisted Superparamagnetic Extraction for Selective Separation of Binary Mixed Proteins
Li, Wensong1,2; Yang, Liangrong1; Zhou, Huacong1,2; Li, Xiaopei1,2; Wang, Fuchun1,2; Yang, Xingfu1; Liu, Huizhou1
2013-11-20
Source PublicationINDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
Volume52Issue:46Pages:16314-16320
AbstractIn this study, gas-assisted superparamagnetic extraction (GASE) with potential large-scale application was proposed for selective separation of multicomponent proteins. Magnetic poly(glycidyl methacrylate)-iminodiacetic acid Zn2+ microspheres (MPMs) for selective separation of bovine serum albumin (BSA) and bovine hemoglobin (BHb) are selected as a model. The feasibility of flotation for concentrating BHb-loaded MPMs from dilute mixed proteins solution was proved. The well-concentrated solution of BHb-loaded MPMs could be achieved by simple adjustment of pH values and ionic strength in low regions, without additive detergent. Impurity BSA had the effects of improving the flotability of BHb-loaded MPMs, acting as foaming agent, but decreasing the enrichment ratio in the GASE process. The flotation conditions and the selective adsorption ones showed strong consistency. Under the optimal conditions, the enrichment ratio of 39 and recovery percentage of 98% was obtained within 1.3 min. Furthermore, the enrichment ratio could be further improved by added flotation steps.
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
Indexed BySCI
Language英语
WOS KeywordBOVINE SERUM-ALBUMIN ; PURIFICATION ; ADSORPTION ; PARTICLES ; FLOTATION
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
WOS IDWOS:000327413500024
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13597
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Wensong,Yang, Liangrong,Zhou, Huacong,et al. Gas-Assisted Superparamagnetic Extraction for Selective Separation of Binary Mixed Proteins[J]. INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,2013,52(46):16314-16320.
APA Li, Wensong.,Yang, Liangrong.,Zhou, Huacong.,Li, Xiaopei.,Wang, Fuchun.,...&Liu, Huizhou.(2013).Gas-Assisted Superparamagnetic Extraction for Selective Separation of Binary Mixed Proteins.INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,52(46),16314-16320.
MLA Li, Wensong,et al."Gas-Assisted Superparamagnetic Extraction for Selective Separation of Binary Mixed Proteins".INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH 52.46(2013):16314-16320.
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