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Peroxidase-like activity of amino-functionalized magnetic nanoparticles and their applications in immunoassay
Yang, Mingzhu1; Guan, Yueping1; Yang, Yu2; Xia, Tingting1; Xiong, Wubin1; Wang, Ning3; Guo, Chen3
2013-09-01
Source PublicationJOURNAL OF COLLOID AND INTERFACE SCIENCE
Volume405Issue:0Pages:291-295
AbstractAmino-functionalized Fe3O4 magnetic nanoparticles with high peroxidase-like activity (MNPs-NH2) are reported in this work. The peroxidase-like activity is approximately the same as naked Fe3O4 magnetic nanoparticles (MNPs). MNPs-NH2 displayed much better thermal stability and pH tolerance than horse-radish peroxidase (HRP). The optimal pH and temperature are approximately pH 4 and 40 degrees C, respectively. We showed that the MNPs-NH2 were successfully applied in a double-antibody sandwich "enzyme"-linked immunosorbent assay to replace of HRP, in which MNPs-NH2 were not only separation carriers, but also detection indicator. (C) 2013 Elsevier Inc. All rights reserved.
KeywordMagnetic Nanoparticle Peroxidase-like Activity Immunoassay
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
Indexed BySCI
Language英语
WOS KeywordFERROMAGNETIC NANOPARTICLES ; PARTICLES ; DELIVERY ; PROGRESS
WOS Research AreaChemistry
WOS SubjectChemistry, Physical
WOS IDWOS:000321598400039
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13598
Collection研究所(批量导入)
Affiliation1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China
2.China Acad Inspect & Quarantine, Beijing 100123, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Yang, Mingzhu,Guan, Yueping,Yang, Yu,et al. Peroxidase-like activity of amino-functionalized magnetic nanoparticles and their applications in immunoassay[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE,2013,405(0):291-295.
APA Yang, Mingzhu.,Guan, Yueping.,Yang, Yu.,Xia, Tingting.,Xiong, Wubin.,...&Guo, Chen.(2013).Peroxidase-like activity of amino-functionalized magnetic nanoparticles and their applications in immunoassay.JOURNAL OF COLLOID AND INTERFACE SCIENCE,405(0),291-295.
MLA Yang, Mingzhu,et al."Peroxidase-like activity of amino-functionalized magnetic nanoparticles and their applications in immunoassay".JOURNAL OF COLLOID AND INTERFACE SCIENCE 405.0(2013):291-295.
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