CAS OpenIR  > 研究所(批量导入)
Insights Into The Working Mechanism and Unfolding Property of Arthrobacter chlorophenolicus Amylosucrase
Liu Ming1,2; Li Ping3; Liu Bin4; Su Ji-Guo5; Wang Cun-Xin4
2013-06-01
Source PublicationPROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
Volume40Issue:6Pages:565-577
AbstractAmylosucrase (AS) is a kind of glucosyltransferase (B.C. 2.4.1.4) belonging to the glycoside hydrolase (GH) family 13. In the presence of an activator polymer, in vitro, AS is able to catalyze the synthesis of an amylose-like polysaccharide composed of only alpha-1,4-linkages using sucrose as the only energy source. Unlike AS, other enzymes responsible for the synthesis of such amylose-like polymers require the addition of expensive nucleotide-activated sugars. These properties make AS an interesting enzyme for industrial applications. In spite of the great application potential, industrial applications of AS were largely hampered by its low stability. In this work, the structure of Arthrobacter chlorophenolicus amylosucrase (AcAS) was modeled according to crystal structures of Neisseria polysaccharea amylosucrase (NpAS) and Deinococcus geothermalis amylosucrase (DgAS). The structural difference of the three AS were investigated for the sake of exploring the working mechanism of AcAS. The dynamics and functional motions of AcAS were also investigated by the Gaussian network model (GNM). Based on GNM, it was found that AcAS can be divided into two regions with different moving directions. Finally, the unfolding property of AcAS was studied by the iterative GNM, and several kinetically weak regions were identified on the basis of the predicted unfolding pathway. These discoveries may be helpful to the industrial development of AS.
KeywordAmylosucrase Homology Modeling Unfolding Gnm Iterative Gnm
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS KeywordGLUCAN-SYNTHESIZING ENZYME ; NEISSERIA-POLYSACCHAREA ; DEINOCOCCUS-GEOTHERMALIS ; FUNCTIONAL EXPRESSION ; GENUS NEISSERIA ; PROTEINS ; GLYCOGEN ; DYNAMICS ; THERMOSTABILITY ; CLASSIFICATION
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000320905700010
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13600
Collection研究所(批量导入)
Affiliation1.Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China
2.Peking Union Med Coll, Beijing 100050, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
4.Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing 100124, Peoples R China
5.Yanshan Univ, Coll Sci, Qinhuangdao 066004, Peoples R China
Recommended Citation
GB/T 7714
Liu Ming,Li Ping,Liu Bin,et al. Insights Into The Working Mechanism and Unfolding Property of Arthrobacter chlorophenolicus Amylosucrase[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2013,40(6):565-577.
APA Liu Ming,Li Ping,Liu Bin,Su Ji-Guo,&Wang Cun-Xin.(2013).Insights Into The Working Mechanism and Unfolding Property of Arthrobacter chlorophenolicus Amylosucrase.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,40(6),565-577.
MLA Liu Ming,et al."Insights Into The Working Mechanism and Unfolding Property of Arthrobacter chlorophenolicus Amylosucrase".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 40.6(2013):565-577.
Files in This Item:
File Name/Size DocType Version Access License
淀粉蔗糖酶AcAS的工作机理与去折叠性质(1646KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu Ming]'s Articles
[Li Ping]'s Articles
[Liu Bin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu Ming]'s Articles
[Li Ping]'s Articles
[Liu Bin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu Ming]'s Articles
[Li Ping]'s Articles
[Liu Bin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.