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Stable gene transfection mediated by polysulfobetaine/PDMAEMA diblock copolymer in salted medium
Dai, Fengying1,2; Liu, Yuan1; Wang, Wei1; Liu, Wenguang1
2013-02-01
Source PublicationJOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Volume24Issue:3Pages:330-343
AbstractCationic polyplexes would aggregate immediately after intravenous injection due to the plasma proteins and high ionic strength. A cationic polyplexes with long-term and salt stability was very important for a systemic gene therapy. In this research, a polysulfobetaine-b-polycation diblock copolymer composed of cationic block of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and zwtterionic block of poly(propylsulfonate dimethylammonium ethylmethacrylate) (PSPE) was conveniently synthesized by atom transfer radical polymerization method to obtain a cationic polymers with long-term and salt stability. The results of agarose gel electrophoresis and transmission electron microscope indicated that copolymerization of PSPE did not compromise the DNA condensation ability of PDMAEMA, meanwhile exhibiting lower cytotoxicity. The effect of salt on the absorbance and particle size of PDMAEMA(100)/DNA and PDMAEMA(100)-PSPEy/DNA complexes was investigated, which showed that PSPE block could increase the resistance of polyplexes against salt-induced aggregation owing to the antielectrolyte effect. In comparison with PDMAEMA homopolymer, PDMAEMA(100)-PSPEy retained more stable gene transfection in a certain range of salt concentration. The expression of red fluorescence protein (RFP) was evaluated by small animal in vivo fluorescence imaging system and the results showed that the expression of RFP was much higher in the mice injected with PDMAEMA(100)-PSPE(20/)pDNA-RFP than with PDMAEMA/pDNA-RFP. Both in vitro and in vivo results suggested that PDMAEMA-PSPE diblock copolymer may be potentially used as a vector for systemic gene therapy.
Keyword2-(DimEthylamino) Ethyl Methacrylate Sulfobetaine Gene Delivery Salt Stability
SubtypeArticle
WOS HeadingsScience & Technology ; Technology ; Physical Sciences
Indexed BySCI
Language英语
WOS KeywordSELF-ASSEMBLED MONOLAYERS ; DNA COMPLEXES ; PROTEIN ADSORPTION ; EXTENDED CIRCULATION ; PHYSICAL STABILITY ; MOLECULAR-WEIGHT ; PLASMID DNA ; IN-VIVO ; DELIVERY ; PEGYLATION
WOS Research AreaEngineering ; Materials Science ; Polymer Science
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials ; Polymer Science
WOS IDWOS:000316011600007
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13642
Collection研究所(批量导入)
Affiliation1.Tianjin Univ, Sch Mat Sci & Engn, Tianjin Key Lab Composite & Funct Mat, Tianjin 300072, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Dai, Fengying,Liu, Yuan,Wang, Wei,et al. Stable gene transfection mediated by polysulfobetaine/PDMAEMA diblock copolymer in salted medium[J]. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,2013,24(3):330-343.
APA Dai, Fengying,Liu, Yuan,Wang, Wei,&Liu, Wenguang.(2013).Stable gene transfection mediated by polysulfobetaine/PDMAEMA diblock copolymer in salted medium.JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,24(3),330-343.
MLA Dai, Fengying,et al."Stable gene transfection mediated by polysulfobetaine/PDMAEMA diblock copolymer in salted medium".JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION 24.3(2013):330-343.
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