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A Simple Technique of Preparing Stable CLEAs of Phenylalanine Ammonia Lyase Using Co-aggregation with Starch and Bovine Serum Albumin
Cui, Jian Dong1,2,3,4; Sun, Li Mei1; Li, Lian Lian1
2013-08-01
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume170Issue:8Pages:1827-1837
AbstractCross-linked enzyme aggregates (CLEAs) have been recently proposed as an alternative to conventional immobilization methods on solid carriers. However, the low cross-linking efficiency causes the major activity loss and instability in the conventional protocol for CLEA preparation. Herein, the effects of bovine serum albumin and starch addition on the cross-linking efficiency of CLEAs of phenylalanine ammonia lyase (PAL) from Rhodotorula glutinis were evaluated. A co-aggregation strategy was developed to improve cross-linking efficiency by adding starch and bovine serum albumin (BSA). CLEAs of PAL prepared in the presence of BSA and starch (PSB-CLEAs) retained 36 % activity, whereas CLEAs prepared without BSA and starch (PAL-CLEAs) retained only 8 % activity of the starting enzyme preparation. Compared with PAL-CLEAs, the thermal stability of PSB-CLEAs has improved considerably, maintaining 30 % residual activity after 4 h of incubation at 70 A degrees C, whereas the PAL-CLEAs have only 13 % residual activity. PSB-CLEAs also exhibited the expected increased stability of PAL against hydrophilic organic solvents, superior operability, and higher storage stability. The proposed technique of preparing CLEAs using co-aggregation with starch and BSA would rank among the potential strategies for efficiently preparing robust and highly stable enzyme aggregates.
KeywordPhenylalanine Ammonia Lyase Rhodotorula Glutinis Cross-linked Enzyme Aggregates Co-aggregation Biocatalysts
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS KeywordRHODOTORULA-GLUTINIS ; ENZYME ; BIOCATALYSTS ; PURIFICATION ; POLYMERS ; ACYLASE
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000322619100003
Citation statistics
Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13652
Collection研究所(批量导入)
Affiliation1.Hebei Univ Sci & Technol, Res Ctr Fermentat Engn Hebei, Coll Biosci & Bioengn, Shijiazhuang 050000, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
3.Tianjin Univ Sci & Technol, Minist Educ, Key Lab Ind Fermentat Microbiol, Tianjin 300457, Peoples R China
4.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Shijiazhuang 050018, Peoples R China
Recommended Citation
GB/T 7714
Cui, Jian Dong,Sun, Li Mei,Li, Lian Lian. A Simple Technique of Preparing Stable CLEAs of Phenylalanine Ammonia Lyase Using Co-aggregation with Starch and Bovine Serum Albumin[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2013,170(8):1827-1837.
APA Cui, Jian Dong,Sun, Li Mei,&Li, Lian Lian.(2013).A Simple Technique of Preparing Stable CLEAs of Phenylalanine Ammonia Lyase Using Co-aggregation with Starch and Bovine Serum Albumin.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,170(8),1827-1837.
MLA Cui, Jian Dong,et al."A Simple Technique of Preparing Stable CLEAs of Phenylalanine Ammonia Lyase Using Co-aggregation with Starch and Bovine Serum Albumin".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 170.8(2013):1827-1837.
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