CAS OpenIR  > 研究所(批量导入)
Preparation, identification, structure, and in vitro anti-obesity effects of protease inhibitors isolated from potato fruit juice
Sun, Ying1,2,3; Liu, Ling2; Jiang, Lian-Zhou1; Zhang, Gui-Feng4; Li, Guo-Ming2; Wu, Na2
2013-08-01
Source PublicationEUROPEAN FOOD RESEARCH AND TECHNOLOGY
Volume237Issue:2Pages:149-157
AbstractIn this study, potato protease inhibitors (PPI) were obtained from industrial potato fruit juice (PFJ) using membrane separation method and ion exchange chromatography. The molecular weight distribution, families, structural characterization, and in vitro anti-proliferative activity of PPI were evaluated in this paper. The molecular weight distribution of PPI was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF-MS). The inhibitor families were identified using high-performance liquid chromatography/mass spectrometry. The structures of the isolated PPI were studied by circular dichroism and fluorescence spectroscopy. Anti-proliferative activity of PPI on 3T3-L1 preadipocytes was investigated by MTT method. The results suggest that ultrafiltration (UF) was more suitable for the isolation of crude proteins from PFJ than nanofiltration. The PPI purified from UF freeze-dried powder were mainly focused on proteins in the region of relative molecular masses (Mr) 20 x 10(3) to 22 x 10(3) with a few low molecular weight proteins, containing protease inhibitor, protease inhibitor II, aspartic protease inhibitor, and other serine protease inhibitor families. The major conformation of PPI was beta-structure, and the tryptophans were mainly located in hydrophobic environments. Furthermore, the structure changed significantly when the temperature reached 80 A degrees C. Moreover, PPI were able to inhibit the proliferation of 3T3-L1 preadipocytes with a 50 % inhibitory concentration of 343.51 mu g/mL. Therefore, PPI may have a potential implication in anti-obesity foods in the future.
KeywordProtease Inhibitors Potato Fruit Juice Isolation Chemical Composition Anti-obesity Effects
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS KeywordEMULSIFYING PROPERTIES ; ADIPOSE-TISSUE ; PROTEINS ; SOLUBILITY ; RECOVERY
WOS Research AreaFood Science & Technology
WOS SubjectFood Science & Technology
WOS IDWOS:000322260200007
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13663
Collection研究所(批量导入)
Affiliation1.Northeast Agr Univ, Coll Food Sci, Harbin 150030, Heilongjiang, Peoples R China
2.China Natl Res Inst Food & Fermentat Ind, Beijing 100027, Peoples R China
3.Qiqihar Univ, Coll Food & Biol Engn, Qiqihar 161006, Heilongjiang, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Sun, Ying,Liu, Ling,Jiang, Lian-Zhou,et al. Preparation, identification, structure, and in vitro anti-obesity effects of protease inhibitors isolated from potato fruit juice[J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY,2013,237(2):149-157.
APA Sun, Ying,Liu, Ling,Jiang, Lian-Zhou,Zhang, Gui-Feng,Li, Guo-Ming,&Wu, Na.(2013).Preparation, identification, structure, and in vitro anti-obesity effects of protease inhibitors isolated from potato fruit juice.EUROPEAN FOOD RESEARCH AND TECHNOLOGY,237(2),149-157.
MLA Sun, Ying,et al."Preparation, identification, structure, and in vitro anti-obesity effects of protease inhibitors isolated from potato fruit juice".EUROPEAN FOOD RESEARCH AND TECHNOLOGY 237.2(2013):149-157.
Files in This Item:
File Name/Size DocType Version Access License
Preparation, identif(363KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Sun, Ying]'s Articles
[Liu, Ling]'s Articles
[Jiang, Lian-Zhou]'s Articles
Baidu academic
Similar articles in Baidu academic
[Sun, Ying]'s Articles
[Liu, Ling]'s Articles
[Jiang, Lian-Zhou]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sun, Ying]'s Articles
[Liu, Ling]'s Articles
[Jiang, Lian-Zhou]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.