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Real Time Monitoring of On-Chip Coenzyme Regeneration with SPR and DPI
Feng, Xiaoyi1,2; Gao, Fei1; Qin, Peiyong2; Ma, Guanghui1; Su, Zhiguo1; Ge, Jia1; Wang, Ping3,4; Zhang, Songping1
2013-02-19
Source PublicationANALYTICAL CHEMISTRY
Volume85Issue:4Pages:2370-2376
AbstractWe report in this work real time characterization of enzyme coenzyme binding by using surface plasmon resonance (SPR) and dual polarization interferometry (DPI) analyses. Results showed that diaphorase (DP) and lactate dehydrogenases (LDH) had distinct binding selectivity and preference over reduced and oxidized states of coenzyme NAD(H). On the basis of that, DP and LDH were chosen as indicator enzymes to distinguish the specific state of surface-bound NAD(H). The transformation between NADH and NAD+ during enzyme-catalyzed redox reactions was therefore transduced into variation in interaction signals as indicated via the binding status of the indicator enzymes as detected with both SPR and DPI. This real time molecule-specific detection strategy revealed quick and direct reflection of the state and reactivity of the coenzyme, promising a unique way of precise molecular interaction analysis.
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
Indexed BySCI
Language英语
WOS KeywordDUAL-POLARIZATION INTERFEROMETRY ; NICOTINAMIDE-ADENINE DINUCLEOTIDE ; PROTEIN ADSORPTION ; AFFINITY CHROMATOGRAPHY ; RESONANCE SPECTROSCOPY ; COFACTOR REGENERATION ; DNA IMMOBILIZATION ; SURFACE ; ELLIPSOMETRY ; DERIVATIVES
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000315326900058
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13726
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Beijing Univ Chem Technol, Coll Life Sci, Beijing 100029, Peoples R China
3.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA
4.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA
Recommended Citation
GB/T 7714
Feng, Xiaoyi,Gao, Fei,Qin, Peiyong,et al. Real Time Monitoring of On-Chip Coenzyme Regeneration with SPR and DPI[J]. ANALYTICAL CHEMISTRY,2013,85(4):2370-2376.
APA Feng, Xiaoyi.,Gao, Fei.,Qin, Peiyong.,Ma, Guanghui.,Su, Zhiguo.,...&Zhang, Songping.(2013).Real Time Monitoring of On-Chip Coenzyme Regeneration with SPR and DPI.ANALYTICAL CHEMISTRY,85(4),2370-2376.
MLA Feng, Xiaoyi,et al."Real Time Monitoring of On-Chip Coenzyme Regeneration with SPR and DPI".ANALYTICAL CHEMISTRY 85.4(2013):2370-2376.
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