CAS OpenIR  > 研究所(批量导入)
Purification and characterization of a cis-epoxysuccinic acid hydrolase from Nocardia tartaricans CAS-52, and expression in Escherichia coli
Wang, Ziqiang1,2; Wang, Yunshan1; Su, Zhiguo1
2013-03-01
Source PublicationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume97Issue:6Pages:2433-2441
AbstractA highly enantioselective cis-epoxysuccinic acid hydrolase from Nocardia tartaricans was purified to electrophoretic homogeneity. The enzyme was purified 184-fold with a yield of 18.8 %. The purified cis-epoxysuccinic acid hydrolase had a monomeric molecular weight of 28 kDa, and its optimum conditions were 37 A degrees C and pH 7-9. With sodium cis-epoxysuccinate as the substrate, Michaelis-Menten enzyme kinetics analysis gave a Km value of 35.71 mM and a Vmax of 2.65 mM min(-1). The enzyme was activated by Ni2+ and Al3+, while strongly inhibited by Fe3+, Fe2+, Cu2+, and Ag+. The cis-epoxysuccinic acid hydrolase gene was cloned, and its open reading frame sequence predicted a protein composed of 253 amino acids. A pET11a expression plasmid carrying the gene under the control of the T7 promoter was introduced into Escherichia coli, and the cis-epoxysuccinic acid hydrolase gene was successfully expressed in the recombinant strains.
KeywordCis-epoxysuccinic Acid Hydrolase Nocardia Tartaricans Prokaryotic Expression Enantioselectivity
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS KeywordAGROBACTERIUM-RADIOBACTER AD1 ; MICROBIAL EPOXIDE HYDROLASES ; ASPERGILLUS-NIGER M200 ; DIRECTED EVOLUTION ; ENANTIOSELECTIVITY ; HYDROLYSIS ; MECHANISM ; CLONING ; ENZYME ; CELLS
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000315650100013
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13741
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Ziqiang,Wang, Yunshan,Su, Zhiguo. Purification and characterization of a cis-epoxysuccinic acid hydrolase from Nocardia tartaricans CAS-52, and expression in Escherichia coli[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(6):2433-2441.
APA Wang, Ziqiang,Wang, Yunshan,&Su, Zhiguo.(2013).Purification and characterization of a cis-epoxysuccinic acid hydrolase from Nocardia tartaricans CAS-52, and expression in Escherichia coli.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(6),2433-2441.
MLA Wang, Ziqiang,et al."Purification and characterization of a cis-epoxysuccinic acid hydrolase from Nocardia tartaricans CAS-52, and expression in Escherichia coli".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.6(2013):2433-2441.
Files in This Item:
File Name/Size DocType Version Access License
Purification and cha(307KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Ziqiang]'s Articles
[Wang, Yunshan]'s Articles
[Su, Zhiguo]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Ziqiang]'s Articles
[Wang, Yunshan]'s Articles
[Su, Zhiguo]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Ziqiang]'s Articles
[Wang, Yunshan]'s Articles
[Su, Zhiguo]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.