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Temperature sensitivity of cellulase adsorption on lignin and its impact on enzymatic hydrolysis of lignocellulosic biomass
Zheng, Yingfu1,2; Zhang, Songping1; Miao, Shida1; Su, Zhiguo1; Wang, Ping3,4
2013-07-10
Source PublicationJOURNAL OF BIOTECHNOLOGY
Volume166Issue:3Pages:135-143
AbstractUnproductive enzyme adsorption is an important factor in addition to steric hindrance of lignin that limits the enzymatic hydrolysis of lignocellulosic biomass. While both are important factors, enzymatic hydrolysis of pretreated biomass is most likely conducted in the presence of certain amount of lignin residues that may not necessarily present accessibility hindrance, but can competitively absorb the enzyme. This paper presents a study with purified lignin samples to elucidate the role of unproductive enzyme adsorption. It appeared that lignin adsorbed cellulase quickly at 4 degrees C with adsorption equilibrium reached within 1 h, similar to that observed for crystalline cellulose. Increasing temperature to 50 degrees C (typical hydrolytic reaction condition) facilitated the rate of cellulase adsorption on cellulose with a peak of adsorption reached at 0.25 h; however, adsorption on lignin was surprisingly slower and took over 12 h to reach equilibrium, which was accompanied with a 10-fold increase in adsorption capacity. Despite the high adsorption capacity of lignin (which is comparable to that of cellulose) at 50 degrees C, the presence of added lignin imposed only minimal impact on the enzyme apparent activity, most likely due to the slow adsorption kinetics of lignin. (c) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
KeywordCellulase Lignin Enzyme Adsorption Steric Hindrance Biomass Recalcitrance Cellulose Hydrolysis
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS KeywordSTEAM PRETREATED SOFTWOOD ; TRICHODERMA-REESEI ; MICROCRYSTALLINE CELLULOSE ; CORN STOVER ; LODGEPOLE PINE ; ENZYMES ; BINDING ; HEMICELLULOSE ; TECHNOLOGIES ; INHIBITION
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000321120200009
Citation statistics
Cited Times:48[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13744
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA
4.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA
Recommended Citation
GB/T 7714
Zheng, Yingfu,Zhang, Songping,Miao, Shida,et al. Temperature sensitivity of cellulase adsorption on lignin and its impact on enzymatic hydrolysis of lignocellulosic biomass[J]. JOURNAL OF BIOTECHNOLOGY,2013,166(3):135-143.
APA Zheng, Yingfu,Zhang, Songping,Miao, Shida,Su, Zhiguo,&Wang, Ping.(2013).Temperature sensitivity of cellulase adsorption on lignin and its impact on enzymatic hydrolysis of lignocellulosic biomass.JOURNAL OF BIOTECHNOLOGY,166(3),135-143.
MLA Zheng, Yingfu,et al."Temperature sensitivity of cellulase adsorption on lignin and its impact on enzymatic hydrolysis of lignocellulosic biomass".JOURNAL OF BIOTECHNOLOGY 166.3(2013):135-143.
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