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Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed
Wang, Feng; Guo, Chen; Liu, Chun-Zhao
2013-12-01
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume171Issue:8Pages:2165-2175
AbstractA magnetically stabilized fluidized bed (MSFB) with the Cu2+-chelated magnetic mesoporous silica nanoparticles (MMSNPs-Cu2+) was established to purify laccase directly from the fermentation broth of Trametes versicolor. The MMSNPs-Cu2+ particles in the MSFB maintained a stable bed expansion of two to threefold at a flow rate of 120-180 cm/h. At the optimal magnetic field intensity of 120 Gs, both the maximal Bodenstein number and the smallest axial dispersion coefficient were achieved, which resulted in a stable fluidization stage. The dynamic binding capacity of laccase in the MSFB decreased from 192.5 to144.3 mg/g when the flow velocity through the bed increased from 44.2 to 69.8 cm/h. The MSFB with MMSNPs-Cu2+ achieved efficient laccase purification from the fermentation broth with 62.4-fold purification of laccase and 108.9 % activity yield. These results provided an excellent platform for the application of these magnetic mesoporous nanoparticles integrated with the MSFB in developing novel protein purification process.
KeywordMagnetically Stabilized Fluidized Bed Magnetic Mesoporous Nanoparticles Axial Liquid Dispersion Laccase Purification
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS KeywordSILICA NANOPARTICLES ; TRAMETES-VERSICOLOR ; PROTEIN ADSORPTION ; AXIAL-DISPERSION ; SEPARATION ; PARTICLES ; BEADS ; CHROMATOGRAPHY ; MICROSPHERES ; PERFORMANCE
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000327494800021
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13745
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Feng,Guo, Chen,Liu, Chun-Zhao. Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2013,171(8):2165-2175.
APA Wang, Feng,Guo, Chen,&Liu, Chun-Zhao.(2013).Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,171(8),2165-2175.
MLA Wang, Feng,et al."Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 171.8(2013):2165-2175.
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