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| Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed | |
| Wang, Feng; Guo, Chen; Liu, Chun-Zhao | |
| 2013-12-01 | |
| Source Publication | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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| Volume | 171Issue:8Pages:2165-2175 |
| Abstract | A magnetically stabilized fluidized bed (MSFB) with the Cu2+-chelated magnetic mesoporous silica nanoparticles (MMSNPs-Cu2+) was established to purify laccase directly from the fermentation broth of Trametes versicolor. The MMSNPs-Cu2+ particles in the MSFB maintained a stable bed expansion of two to threefold at a flow rate of 120-180 cm/h. At the optimal magnetic field intensity of 120 Gs, both the maximal Bodenstein number and the smallest axial dispersion coefficient were achieved, which resulted in a stable fluidization stage. The dynamic binding capacity of laccase in the MSFB decreased from 192.5 to144.3 mg/g when the flow velocity through the bed increased from 44.2 to 69.8 cm/h. The MSFB with MMSNPs-Cu2+ achieved efficient laccase purification from the fermentation broth with 62.4-fold purification of laccase and 108.9 % activity yield. These results provided an excellent platform for the application of these magnetic mesoporous nanoparticles integrated with the MSFB in developing novel protein purification process. |
| Keyword | Magnetically Stabilized Fluidized Bed Magnetic Mesoporous Nanoparticles Axial Liquid Dispersion Laccase Purification |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | SILICA NANOPARTICLES ; TRAMETES-VERSICOLOR ; PROTEIN ADSORPTION ; AXIAL-DISPERSION ; SEPARATION ; PARTICLES ; BEADS ; CHROMATOGRAPHY ; MICROSPHERES ; PERFORMANCE |
| WOS Research Area | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS Subject | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| WOS ID | WOS:000327494800021 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/13745 |
| Collection | 研究所（批量导入） |
| Affiliation | Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Feng,Guo, Chen,Liu, Chun-Zhao. Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2013,171(8):2165-2175. |
| APA | Wang, Feng,Guo, Chen,&Liu, Chun-Zhao.(2013).Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,171(8),2165-2175. |
| MLA | Wang, Feng,et al."Functional Magnetic Mesoporous Nanoparticles for Efficient Purification of Laccase from Fermentation Broth in Magnetically Stabilized Fluidized Bed".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 171.8(2013):2165-2175. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Functional Magnetic （295KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | Application Full Text | |
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