CAS OpenIR  > 研究所(批量导入)
Determination of leakage from antibody adsorbent: composition analysis and pH effect
Zhao, Lan; Liu, Yongdong; Wang, Yinjue; Huang, Yongdong; Li, Xiunan; Li, Yan; Ma, Guanghui; Su, Zhiguo
2013-09-01
Source PublicationBIOMEDICAL CHROMATOGRAPHY
Volume27Issue:9Pages:1089-1091
AbstractTo study the leakage at different solution pH values, IgG Sepharose 6FF (R), a commercially available immunoadsorbent, was used as a model. The leaked substance consists of three parts: (1) ligands and its fragments; (2) ligands plus matrix fragments in which ligands are chemically attached to the adsorbent matrix; and (3) matrix fragments. Buffer solution pH values had a great effect on both the kinetics and the amount of ligand leakage. Cross-linking of the adsorbent matrix could reduce both matrix leakage and antibody leakage at pH 3.0, but its effect was limited at pH 11.0 for ligand leakage. Copyright (C) 2013 John Wiley & Sons, Ltd.
KeywordImmunoadsorbent Human Immunoglobulin g Ligand Leakage Matrix Leakage Ph-dependent
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Language英语
WOS KeywordFAST-FLOW ; CHROMATOGRAPHY ; IMMUNOADSORBENTS ; STABILITY
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000322717100001
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13762
Collection研究所(批量导入)
AffiliationChinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Lan,Liu, Yongdong,Wang, Yinjue,et al. Determination of leakage from antibody adsorbent: composition analysis and pH effect[J]. BIOMEDICAL CHROMATOGRAPHY,2013,27(9):1089-1091.
APA Zhao, Lan.,Liu, Yongdong.,Wang, Yinjue.,Huang, Yongdong.,Li, Xiunan.,...&Su, Zhiguo.(2013).Determination of leakage from antibody adsorbent: composition analysis and pH effect.BIOMEDICAL CHROMATOGRAPHY,27(9),1089-1091.
MLA Zhao, Lan,et al."Determination of leakage from antibody adsorbent: composition analysis and pH effect".BIOMEDICAL CHROMATOGRAPHY 27.9(2013):1089-1091.
Files in This Item:
File Name/Size DocType Version Access License
Determination of lea(120KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhao, Lan]'s Articles
[Liu, Yongdong]'s Articles
[Wang, Yinjue]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhao, Lan]'s Articles
[Liu, Yongdong]'s Articles
[Wang, Yinjue]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhao, Lan]'s Articles
[Liu, Yongdong]'s Articles
[Wang, Yinjue]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.