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Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization
Liu, Ying1; Guo, Chen2; Sun, Xi-Tong2; Liu, Chun-Zhao1
2013-08-01
Source PublicationBIORESOURCE TECHNOLOGY
Volume142Issue:0Pages:415-419
AbstractYarrowia lipolytica lipase (YLL) demonstrated an (R)-enantiopreference for efficient resolution of (R,S)-2-octanol. The activity, enantioselectivity, the ratio of substrate to enzyme, acetaldehyde tolerance, and operational stability of YLL were improved by an integrated strategy of interfacial activation, bioimprinting, and immobilization. In comparison with the control, both the enzymatic activity and enantioselectivity increased by a factor of 8.85 and 2.75 by the integrated strategy, respectively. Fifty-one percentage of conversion with 220 of enantioselectivity was obtained using the immobilized YLL prepared by the integrated strategy at a ratio of 104 of substrate to enzyme loaded. The immobilized YLL retained 97% of its initial activity without a decrease in enantioselectivity after 10 successive reuse cycles. Together these results will result in a promising strategy with the YYL for efficient resolution of (R,S)-2-octanol in practice. (C) 2013 Elsevier Ltd. All rights reserved.
KeywordYarrowia Lipolytica Lipase Bioimprinting Immobilization Resolution 2-octanol
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
Indexed BySCI
Language英语
WOS KeywordPOLYUNSATURATED FATTY-ACIDS ; MACROPOROUS RESIN ; ACETALDEHYDE ; ADSORPTION ; STABILIZATION ; PROTEINS ; ENZYMES ; BINDING
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000322292800055
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13768
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Liu, Ying,Guo, Chen,Sun, Xi-Tong,et al. Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization[J]. BIORESOURCE TECHNOLOGY,2013,142(0):415-419.
APA Liu, Ying,Guo, Chen,Sun, Xi-Tong,&Liu, Chun-Zhao.(2013).Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization.BIORESOURCE TECHNOLOGY,142(0),415-419.
MLA Liu, Ying,et al."Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization".BIORESOURCE TECHNOLOGY 142.0(2013):415-419.
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