CAS OpenIR  > 研究所(批量导入)
Air bubble resistant and disposable microPCR chip with a portable and programmable device for forensic test
Xu, Jiandong1; Lv, Xuefei2; Wei, Yuping1; Zhang, Liang1,3; Li, Rui2; Deng, Yulin2; Xu, Xia1
2015-06-01
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
Volume212Issue:JUNPages:472-480
Abstract

Due to the difficulty in sample introduction, the presence of air bubbles and the complicated fabrication with high cost, there are no commercial products of microfluidic polymerase chain reaction (microPCR) on the market yet. In this study, an air bubble resistant and disposable microPCR chip was developed. A simple fabrication method using Norland Optical Adhesive 81 (NOA81) as the substrates was employed to prepare the microPCR chip. The temperature of the microPCR chip was controlled using a self-developed portable and programmable PCR apparatus. The simulation results indicate that the developed microPCR chip has good performance in temperature stability even in the presence of air bubbles. The DNA was successfully amplified without adding bovine serum albumin, implicating that the developed microPCR chip has low adsorption of DNA polymerase. The good performance of microPCR chip attributed to the property of N0A81, the fabrication method and the irreversible sealing. The portable and programmable PCR device combined with the disposable microPCR chip is suitable for the amplification of both short and long DNA fragments and Short Tandem Repeats (STRs). It offers a practical approach for portable forensic test, combining the cost benefits of small volume reagent with the disposable polymer microPCR chip. (C) 2015 Elsevier B.V. All rights reserved.

KeywordDispoable Micropcr Chip Noa81 Portable And Programmable Pcr Device Air Bubble Resistant
SubtypeArticle
Subject AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
DOI10.1016/j.snb.2015.02.064
Indexed BySCI
Language英语
WOS KeywordReal-time Pcr ; Microfluidic Devices ; Dna Amplification ; Microchip ; System ; Lab
Funding Projectkey project in the national science & technology pillar program during the eleventh five-year plan period [2009BAK59B02]
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000351017700061
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13773
Collection研究所(批量导入)
Corresponding AuthorDeng, Yulin
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Xu, Jiandong,Lv, Xuefei,Wei, Yuping,et al. Air bubble resistant and disposable microPCR chip with a portable and programmable device for forensic test[J]. SENSORS AND ACTUATORS B-CHEMICAL,2015,212(JUN):472-480.
APA Xu, Jiandong.,Lv, Xuefei.,Wei, Yuping.,Zhang, Liang.,Li, Rui.,...&Xu, Xia.(2015).Air bubble resistant and disposable microPCR chip with a portable and programmable device for forensic test.SENSORS AND ACTUATORS B-CHEMICAL,212(JUN),472-480.
MLA Xu, Jiandong,et al."Air bubble resistant and disposable microPCR chip with a portable and programmable device for forensic test".SENSORS AND ACTUATORS B-CHEMICAL 212.JUN(2015):472-480.
Files in This Item:
File Name/Size DocType Version Access License
Air bubble resistant(3175KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xu, Jiandong]'s Articles
[Lv, Xuefei]'s Articles
[Wei, Yuping]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xu, Jiandong]'s Articles
[Lv, Xuefei]'s Articles
[Wei, Yuping]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xu, Jiandong]'s Articles
[Lv, Xuefei]'s Articles
[Wei, Yuping]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.