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Novel konjac glucomannan microcarriers for anchorage-dependent animal cell culture
Sun, Lijing1; Xiong, Zhidong1,2; Zhou, Weiqing1,3; Liu, Ruina1; Yan, Xingran3; Li, Juan3; An, Wenqi4; Yuan, Guoqiang4; Ma, Guanghui1,3; Su, Zhiguo1,3
2015-04-15
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume96Issue:APRPages:46-54
Abstract

A new type of microcarrier was prepared for anchorage-dependent animal cell culture. Similar to the commercial product Cytodex 1, the newcomer is also spherical in micro-size range with ionic group DEAE, but the matrix is konjac glucomannan (KGM) from a natural plant. The optimal anion exchange capacity (AEC) was found to be 2.1 to 2.4 mmol/g dry microspheres. Five cell lines were cultured in static mode with the KGM microcarriers, including Vero, CHO-K1, MDCK, Wish and L929 cells. These cells adhered well and grew to high cell density comparable with or even better than those did on Cytodex 1. A further comparison was made with Vero cell culture in spinner mode. It was found that Vero cells achieved more than 2 x 10(6) cells/ml, and showed faster cell adhesion and higher specific growth rate on the KGM microcarriers than on Cytodex 1. In 5 L bioreactor perfusion culture, Vero cells grew above 7.0 x 10(6) cells/ml and the rabies virus titer was about 7.7 IgLD(50)/ml, compared to Cytodex 1 in the same culture condition. The results suggest that the new type of microcarrier could be a promising candidate for various cell cultures. (C) 2014 Elsevier B.V. All rights reserved.

KeywordKonjac Glucomannan Microcarriers Animal Cell Culture Anion Exchange Capacity Large-scale Cultivation Glucose
SubtypeArticle
Subject AreaBiotechnology & Applied Microbiology ; Engineering
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.bej.2014.12.012
Indexed BySCI
Language英语
WOS KeywordVero Cells ; Rabies Virus ; Japanese Encephalitis ; Microrough Surface ; Stem-cells ; In-vitro ; Growth ; Attachment ; Bioreactor ; Hydrogel
Funding ProjectNational Key Basic Research Program of China (973 Program) [2013CB733600] ; National High Technology Research and Development Program of China (863 Program) [2012AA021202, 2012AA02A406, 2014AA021006] ; National Key Scientific Instrument and Equipment Development Project [2013YQ14040508] ; National Natural Science Foundation of China [21336010]
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000350776500007
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13779
Collection研究所(批量导入)
Corresponding AuthorSu, Zhiguo
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Natl Engn Res Ctr Biotechnol Beijing, Beijing 100190, Peoples R China
4.Hualan Biol Bacterin Co Ltd, Xinxiang 453003, Peoples R China
Recommended Citation
GB/T 7714
Sun, Lijing,Xiong, Zhidong,Zhou, Weiqing,et al. Novel konjac glucomannan microcarriers for anchorage-dependent animal cell culture[J]. BIOCHEMICAL ENGINEERING JOURNAL,2015,96(APR):46-54.
APA Sun, Lijing.,Xiong, Zhidong.,Zhou, Weiqing.,Liu, Ruina.,Yan, Xingran.,...&Su, Zhiguo.(2015).Novel konjac glucomannan microcarriers for anchorage-dependent animal cell culture.BIOCHEMICAL ENGINEERING JOURNAL,96(APR),46-54.
MLA Sun, Lijing,et al."Novel konjac glucomannan microcarriers for anchorage-dependent animal cell culture".BIOCHEMICAL ENGINEERING JOURNAL 96.APR(2015):46-54.
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