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Characterization of a new endo-type alginate lyase from Vibrio sp W13
Zhu, Benwei1,2; Tan, Haidong1; Qin, Yuqi3; Xu, Qingsong1; Du, Yuguang1,4; Yin, Heng1

A gene, encoding a new alginate lyase Algb, was identified and cloned from marine bacterium Vibrio sp. W13. The recombinant alginate lyase was characterized followed by being purified on Ni-NTA Sepharose. It exhibited the highest activity (457 U/mg) at pH 8.0 and 30 degrees C Interestingly, Algb possessed broader substrate specificity. It showed activities toward both polyM (poly beta-D-mannuronate) and polyG (poly alpha-L-guluronate). Furthermore, K-m values of Algb toward alginate (0.67 mg/ml) and polyMG (0.50 mg/ml) are lower than those toward polyG (1.04 mg/ml) and polyM (6.90 mg/ml). The TLC and ESI-MS analysis suggested that Algb mainly released oligosaccharides with DP of 2-5 from the four kinds of substrates in an endolytic manner. Therefore, it may be a potent tool to produce alginate oligosaccharides with low DP. (C) 2015 Elsevier B.V. All rights reserved.

KeywordAlginate Lyase Polysaccharide Lyase Family 7 Vibrio Sp.
Subject AreaBiochemistry & Molecular Biology
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
WOS KeywordOligoalginate Lyase ; Sequence-analysis ; Saccharophagus Degradans ; Cystic-fibrosis ; Raw264.7 Cells ; Cloning ; Purification ; Oligosaccharides ; Polysaccharide ; Depolymerization
Funding ProjectNational Key Technology Support Program [2013BAB01B01] ; Special Fund for Marine Scientific Research [201305015-2] ; National Natural Science Foundation of China [31100203] ; Development Fund for Collaborative Innovation Center of Glycoscience of Shandong University ; Ocean Public Welfare Scientific Research Project, State Oceanic Administration of China [201405003]
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000352332700044
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Document Type期刊论文
Corresponding AuthorDu, Yuguang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250100, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Benwei,Tan, Haidong,Qin, Yuqi,et al. Characterization of a new endo-type alginate lyase from Vibrio sp W13[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,75(APR):330-337.
APA Zhu, Benwei,Tan, Haidong,Qin, Yuqi,Xu, Qingsong,Du, Yuguang,&Yin, Heng.(2015).Characterization of a new endo-type alginate lyase from Vibrio sp W13.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,75(APR),330-337.
MLA Zhu, Benwei,et al."Characterization of a new endo-type alginate lyase from Vibrio sp W13".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 75.APR(2015):330-337.
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