CAS OpenIR  > 研究所(批量导入)
Preparation of PEGA grafted poly(chloromethylstyrene-co-ethylene glycol dimethacrylate) monolith for high-efficiency solid phase peptide synthesis under continuous flow techniques
Zhang, Rongyue1,2; Li, Qiang2; Huang, Yongdong2; Zhao, Lan2; Ye, Peili2; Ma, Guanghui2; Su, Zhiguo2
2015-03-20
Source PublicationPOLYMER
ISSN0032-3861
Volume61Issue:MARPages:115-122
Abstract

The polymer monolith for solid-phase synthesis was prepared through in situ copolymerization of chloromethylstyrene and ethylene glycol dimethacrylate (PCMS-EDMA), and the obtained monolith was grafted by poly (ethylene glycol) acrylate monomer via activators generated by electron transfer atom transfer radical polymerization (AGET ATRP). The novel monolith was highly crosslinked and showed no detectable swelling in both polar and nonpolar solvents (e.g. dichloromethane, dimethylformamide, tetrahydrofuran, acetonitrile, and methanol). The grafted monolith increased the number of functional groups in the range of 0.32-0.85 mmol/g, which resulted from side groups in each grafting polymer chain. Meanwhile, the grafted monolith showed good permeability and mechanical strength under the flow-through conditions. This monolith was derived into Wang resin and used in the synthesis of a difficult sequence-acyl carrier protein fragment 65-74 (ACP 65-74) in a new designed continuous flow equipment. The yield and purity of the crude peptide acquired from the grafted monolith reached 82% and 92%, respectively, which were higher than those by the ungrafted monolith (51% and 60%, respectively) or commercial Wang resin (51% and 61%, respectively). The synthetic efficiency on the grafted monolith in the continuous flow technique was 4-5 folds higher than on Wang resins in the manually operation conditions. Therefore, this monolithic resin showed potential effects in production scale-up. (C) 2015 Elsevier Ltd. All rights reserved.

KeywordMonolith Solid Phase Peptide Synthesis Continuous Flow Technique
SubtypeArticle
Subject AreaPolymer Science
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1016/j.polymer.2015.01.071
Indexed BySCI
Language英语
WOS KeywordTransfer Radical Polymerization ; Affinity-chromatography ; Raft Polymerization ; Cross-linking ; Separation ; Chemistry ; Columns ; Support ; Resin ; Methacrylate
Funding ProjectNational Natural Science Foundation of China [51103158, 21106163, 21306206] ; National Key Technology R&D Program of the Ministry of Science and Technology [2012BA144G01, 2013BAB01B03] ; National High Technology Research and Development Program of China (863 Program) [2014AA021005]
WOS Research AreaPolymer Science
WOS SubjectPolymer Science
WOS IDWOS:000352118200014
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13802
Collection研究所(批量导入)
Corresponding AuthorZhang, Rongyue
Affiliation1.Beijing Inst Petrochem Technol, Dept Chem Engn, Beijing 102617, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Rongyue,Li, Qiang,Huang, Yongdong,et al. Preparation of PEGA grafted poly(chloromethylstyrene-co-ethylene glycol dimethacrylate) monolith for high-efficiency solid phase peptide synthesis under continuous flow techniques[J]. POLYMER,2015,61(MAR):115-122.
APA Zhang, Rongyue.,Li, Qiang.,Huang, Yongdong.,Zhao, Lan.,Ye, Peili.,...&Su, Zhiguo.(2015).Preparation of PEGA grafted poly(chloromethylstyrene-co-ethylene glycol dimethacrylate) monolith for high-efficiency solid phase peptide synthesis under continuous flow techniques.POLYMER,61(MAR),115-122.
MLA Zhang, Rongyue,et al."Preparation of PEGA grafted poly(chloromethylstyrene-co-ethylene glycol dimethacrylate) monolith for high-efficiency solid phase peptide synthesis under continuous flow techniques".POLYMER 61.MAR(2015):115-122.
Files in This Item:
File Name/Size DocType Version Access License
Preparation of PEGA (1447KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Rongyue]'s Articles
[Li, Qiang]'s Articles
[Huang, Yongdong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Rongyue]'s Articles
[Li, Qiang]'s Articles
[Huang, Yongdong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Rongyue]'s Articles
[Li, Qiang]'s Articles
[Huang, Yongdong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.