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Optimization protocols and improved strategies of cross-linked enzyme aggregates technology: current development and future challenges
Cui, Jian Dong1,2,3; Jia, Shi Ru3
2015-03-01
Source PublicationCRITICAL REVIEWS IN BIOTECHNOLOGY
ISSN0738-8551
Volume35Issue:1Pages:15-28
Abstract

Cross-linked enzyme aggregate (CLEA) technology has been regarded as an effective carrier-free immobilization method. This method is very attractive due to its simplicity and robustness, as well as for the possibility of using the crude enzyme extract and the opportunity to co-immobilize multiple different enzymes. The resulting CLEAs generally exhibit high catalyst productivities, improved storage and operational stability and are easy to recycle. Nowadays, although the technology has been applied to various enzymes, some undesirable properties have limited its further application. To overcome these limitations, novel strategies have been developing in recent years. This mini-review focuses on process optimization, new improved strategies and the latest advances on CLEAs technology.

KeywordBiocatalysis Cross-linked Enzyme Aggregates Enzyme Immobilization Improved Strategies Process Optimization
SubtypeReview
Subject AreaBiotechnology & Applied Microbiology
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.3109/07388551.2013.795516
Indexed BySCI
Language英语
WOS KeywordPenicillin-g Acylase ; Phenylalanine Ammonia-lyase ; Endocrine-disrupting Chemicals ; Bovine Serum-albumin ; Thermophilic Enzymes ; Stable Biocatalysts ; Industrial Enzymes ; Carboxypeptidase-a ; Candida-antarctica ; Mesoporous Silica
Funding ProjectNational Natural Science Foundation of China (NSFC) [21072041] ; Foundation of Tianjin Key Laboratory of Industrial Microbiology (Tianjin University of Science and Technology), P. R. China [2012IM004] ; Foundation of Hebei University of Science and technology for Distinguished Young Scientists
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000350017200002
Citation statistics
Cited Times:118[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/13823
Collection研究所(批量导入)
Corresponding AuthorCui, Jian Dong
Affiliation1.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Res Ctr Fermentat Engn Hebei, Shijiazhuang 050018, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
3.Tianjin Univ Sci & Technol, Minist Educ, Key Lab Ind Microbiol, Tianjin, Peoples R China
Recommended Citation
GB/T 7714
Cui, Jian Dong,Jia, Shi Ru. Optimization protocols and improved strategies of cross-linked enzyme aggregates technology: current development and future challenges[J]. CRITICAL REVIEWS IN BIOTECHNOLOGY,2015,35(1):15-28.
APA Cui, Jian Dong,&Jia, Shi Ru.(2015).Optimization protocols and improved strategies of cross-linked enzyme aggregates technology: current development and future challenges.CRITICAL REVIEWS IN BIOTECHNOLOGY,35(1),15-28.
MLA Cui, Jian Dong,et al."Optimization protocols and improved strategies of cross-linked enzyme aggregates technology: current development and future challenges".CRITICAL REVIEWS IN BIOTECHNOLOGY 35.1(2015):15-28.
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