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LPreparation and Characterization of a Thermoresponsive Gigaporous Bioseparation Medium
Qu, Jian-bo1; Chen, Yan-li1; Huan, Guan-sheng1; Zhou, Wei-qing2; Zhang, Xiao-yun1

Gigaporous PS microspheres, prepared in a previous study, were hydrophilized with modified agarose (Agap-PS), and then a thermoresponsive gigaporous bioseparation medium (Agap-PS-PNIPAM) was prepared through a two-step reaction. Bromoacetyl groups were coupled on Agap-PS through acylation to obtain a solid atom transfer radical polymerization (ATRP) initiator (Agap-PS-Br), and poly(N-isopropylacrylamide) (PNIPAM) brushes were grafted onto Agap-PS-Br via ATRP afterwards. The effects of ligands, catalysts, solvent and temperature on NIPAM solution ATRP were investigated in detail, and the amount of PNIPAM grafted on Agap-PS-Br reached to 15. 07 mg/m(2) under optimal conditions. Agap-PS-PNIPAM was characterized by Fourier transform infrared (FTIR) spectra, scanning electron microscope (SEM), mercury porosimetry measurements, laser confocal scanning microscope (LCSM), and protein adsorption experiments. Results showed that thermoresponsive PNIPAM brushes were successfully grafted on the PS microspheres and that the Agap-PS-PNIPAM had good thermo-responsibility. Meanwhile, the gigaporous structure of PS microspheres was well maintained. After modification, nonspecific adsorption of proteins on PS microspheres was greatly reduced. The adsorption amount of bovine serum albumin (BSA) on Agap-PS-PNIPAM at 40 degrees C was 2. 69 times to that at 25 degrees C due to the phase transition of PNIPAM brushes from hydrophilicity to hydrophobicity. Flow experiments showed that the Agap-PS-PNIPAM column had low backpressure, good permeability and mechanical stability. Similarity, the bed permeability (K) of Agap-PS-PNIPAM column at 40 degrees C increased by 15. 7% than that at 25 degrees C owing to the shrinking of PNIPAM brushes. All results indicate that Agap-PS-PNIPAM has great potentials in green high-speed protein chromatography.

KeywordGigaporous Polystyrene Microspheres Hydrophilization Atom Transfer Radical Polymerization Thermoresponsive Polymer Brush N-isopropylacrylamide
Subject AreaPolymer Science
WOS HeadingsScience & Technology ; Physical Sciences
Indexed BySCI
WOS KeywordTemperature-responsive Chromatography ; Transfer Radical Polymerization ; Separation ; Microspheres ; Exchange ; Polymers ; Matrix ; Silica
WOS Research AreaPolymer Science
WOS SubjectPolymer Science
WOS IDWOS:000352004400008
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Document Type期刊论文
Corresponding AuthorQu, Jian-bo
Affiliation1.China Univ Petr East China, Ctr Bioengn & Biotechnol, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Qu, Jian-bo,Chen, Yan-li,Huan, Guan-sheng,et al. LPreparation and Characterization of a Thermoresponsive Gigaporous Bioseparation Medium[J]. ACTA POLYMERICA SINICA,2015(1):57-64.
APA Qu, Jian-bo,Chen, Yan-li,Huan, Guan-sheng,Zhou, Wei-qing,&Zhang, Xiao-yun.(2015).LPreparation and Characterization of a Thermoresponsive Gigaporous Bioseparation Medium.ACTA POLYMERICA SINICA(1),57-64.
MLA Qu, Jian-bo,et al."LPreparation and Characterization of a Thermoresponsive Gigaporous Bioseparation Medium".ACTA POLYMERICA SINICA .1(2015):57-64.
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一种温敏型超大孔生物分离介质的制备及表征(1888KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
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