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"Ready-to-use" hollow nanofiber membrane-based glucose testing strips
Ji, Xiaoyuan1; Su, Zhiguo1,2; Wang, Ping1,3,4; Ma, Guanghui1; Zhang, Songping1,2
2014-12-21
Source PublicationANALYST
ISSN0003-2654
Volume139Issue:24Pages:6467-6473
Abstract

A novel "ready-to-use" glucose test strip based on a polyurethane hollow nanofiber membrane was fabricated through facile co-axial electrospinning. By utilizing glucose oxidase and horseradish peroxidase in the core-phase solution, and a chromogenic agent either in the core solution (in which case 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) was used) or in the shell-phase solution (in which case o-dianisidine was used) for co-axial electrospinning, in situ co-encapsulation of the two enzymes within the hollow nano-chamber and incorporation of chromogenic agents either inside the nano-chamber or in the shell of the hollow nanofibers was realized. Such unique "all-in-one" feature enabled the prepared hollow nanofiber membrane-based test strips to be applied either as colorimetric sensors in solution or as an optical biosensor operated in the "dip-and-read" mode. When used as a colorimetric biosensor in solution, the test strip with o-dianisidine as chromogenic agent shows an excellent linear response range between 0.01 mM to 20 mM and a high apparent lumped activity recovery of 62.1% as compared to the reaction rate of the free bi-enzyme system. While the activity recovery of the test strip with ABTS as chromogenic agent is only 18.0%, and the test strip is found to be unstable due to spontaneous-oxidation of the ABTS. The o-dianisidine test strip was also applied as an optical biosensor, visible rufous color was quickly developed on the surface of the membrane upon dropping 10 mu L of glucose sample, and an excellent correlation between differential diffusive reflectance of the test strip at 440 nm and glucose concentration was obtained in the range of 0.5-50 mM. The test strips also exhibited excellent long-term storage stability with a half-life at 25 degrees C as long as four months.

KeywordElectrochemical Biosensors Enzyme Immobilization Composite Nanofibers Gold Nanoparticles Carbon Nanofiber Oxidase Electrode Nanocomposite Nanotube Polymer
SubtypeArticle
Subject AreaChemistry
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1039/c4an01354a
Indexed BySCI
Language英语
WOS KeywordElectrochemical Biosensors ; Enzyme Immobilization ; Composite Nanofibers ; Gold Nanoparticles ; Carbon Nanofiber ; Oxidase ; Electrode ; Nanocomposite ; Nanotube ; Polymer
Funding ProjectNational Natural Science Foundation of China [21376249, 21336010, 20976180] ; 973 Program [2013CB733604]
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000345265300020
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/15243
Collection研究所(批量导入)
Corresponding AuthorZhang, Songping
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China
3.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA
4.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA
Recommended Citation
GB/T 7714
Ji, Xiaoyuan,Su, Zhiguo,Wang, Ping,et al. "Ready-to-use" hollow nanofiber membrane-based glucose testing strips[J]. ANALYST,2014,139(24):6467-6473.
APA Ji, Xiaoyuan,Su, Zhiguo,Wang, Ping,Ma, Guanghui,&Zhang, Songping.(2014)."Ready-to-use" hollow nanofiber membrane-based glucose testing strips.ANALYST,139(24),6467-6473.
MLA Ji, Xiaoyuan,et al.""Ready-to-use" hollow nanofiber membrane-based glucose testing strips".ANALYST 139.24(2014):6467-6473.
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