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Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli
Bai, Bing1,2; Zhou, Jie-min1,2; Yang, Mao-hua1; Liu, Yi-lan1,2; Xu, Xiao-hui1,2; Xing, Jian-min1
2015-06-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume185Pages:56-61
AbstractIn this study, microbial production of succinic acid from macroalgae (i.e., Laminaria japonica) was investigated for the first time. The engineered Escherichia coli BS002 exhibited higher molar yield of succinic acid on mannitol (1.39 +/- 0.01 mol/mol) than glucose (1.01 +/- 0.05 mol/mol). After pretreatment and enzymatic hydrolysis, L. japonica hydrolysate was mainly glucose (10.31 +/- 0.32 g/L) and mannitol (10.12 +/- 0.17 g/L), which was used as the substrate for succinic acid fermentation with the recombinant BS002. A final 17.44 +/- 0.54 g/L succinic acid was obtained from the hydrolysate after 72 h dual-phase fermentation. The yield was as high as 1.24 +/- 0.08 mol/mol total sugar, which reached 73% of the maximum theoretical yield. The results demonstrate that macroalgae biomass represents a novelty and economical alternative feedstock for biochemicals production. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordEscherichia Coli Laminaria Japonica Macroalgae Mannitol Succinic Acid
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2015.02.081
Indexed BySCI
Language英语
WOS KeywordCONSERVING PATHWAYS ; SEAWEED HYDROLYSATE ; LAMINARIA-JAPONICA ; NADH/NAD(+) RATIO ; CARBON-SOURCES ; NAD(H) POOL ; EVOLUTION ; PLATFORM ; SACCHARIFICATION ; OVEREXPRESSION
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000353190500009
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/16739
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Bai, Bing,Zhou, Jie-min,Yang, Mao-hua,et al. Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli[J]. BIORESOURCE TECHNOLOGY,2015,185:56-61.
APA Bai, Bing,Zhou, Jie-min,Yang, Mao-hua,Liu, Yi-lan,Xu, Xiao-hui,&Xing, Jian-min.(2015).Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli.BIORESOURCE TECHNOLOGY,185,56-61.
MLA Bai, Bing,et al."Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli".BIORESOURCE TECHNOLOGY 185(2015):56-61.
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