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Dual-functional OPH-immobilized polyamide nanofibrous membrane for effective organophosphorus toxic agents protection
Yan, Xiao-Yu1,2; Jiang, Yan-Jun1; Zhang, Song-Ping2,3; Gao, Jing1; Zhang, Yu-Fei2
2015-06-01
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume98Issue:15Pages:47-55
AbstractIn both civilian and military domains, the increasing use of highly toxic organophosphates (OPs) has created an urgent demand for developing an effective method that could protect the human body from OP poisoning. In this study, a novel dual-functional protecting material with both OP filtration and degradation functions was designed and fabricated. The filtration function was endowed by the electrospinning polyamide 66 (Nylon 66, PA-66) nanofibrous membrane, while the OP degradation function was based on the immobilization of organophosphorus hydrolase (OPH) on membrane through glutaraldehyde (GA) crosslinking. A systematic study of the relationship between the membrane structure, filtration ability and OP degradation activity revealed that PA-66 nanofibrous enzyme with 20 mu m thickness exhibited perfect uniform morphology, and the particle filtration efficiency was over 99%. The nanofibrous enzyme exhibited a specific activity of 41 U/g; the enzyme activity recovery was 53.3%, and the methyl parathion (MP) degradation efficiency was 40%. Moreover, the nanofibrous enzyme expressed excellent organic solvent stability; approximately 70% of its initial activity was retained after incubation in xylene solvent for 24 h at 25 degrees C. The residual activity was 40% after being stored for 30 d at 25 degrees C After 10 repeated uses, the residual activity of the nanofibrous membrane was 37%. This study demonstrated the application potential of dual-functional PA-66 nanofibrous enzyme in biochemical protection, aimed at various military and civilian applications. (C) 2015 Elsevier B.V. All rights reserved.
KeywordNanofibrous Membrane Immobilized Enzymes Biodegradation Particle Filtration Enzyme Biocatalysis Kinetic Parameters
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.bej.2015.02.022
Indexed BySCI
Language英语
WOS KeywordELECTROSPUN NANOFIBERS ; ENZYME IMMOBILIZATION ; PSEUDOMONAS-DIMINUTA ; POLYMER NANOFIBERS ; NERVE AGENTS ; HYDROLYSIS ; PHOSPHOTRIESTERASE ; HYDROLASE ; FIBERS ; NANOCOMPOSITES
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000353008000006
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/16742
Collection生化工程国家重点实验室
Affiliation1.Hebei Univ Technol, Sch Chem Engn & Technol, Tianjin 300130, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China
Recommended Citation
GB/T 7714
Yan, Xiao-Yu,Jiang, Yan-Jun,Zhang, Song-Ping,et al. Dual-functional OPH-immobilized polyamide nanofibrous membrane for effective organophosphorus toxic agents protection[J]. BIOCHEMICAL ENGINEERING JOURNAL,2015,98(15):47-55.
APA Yan, Xiao-Yu,Jiang, Yan-Jun,Zhang, Song-Ping,Gao, Jing,&Zhang, Yu-Fei.(2015).Dual-functional OPH-immobilized polyamide nanofibrous membrane for effective organophosphorus toxic agents protection.BIOCHEMICAL ENGINEERING JOURNAL,98(15),47-55.
MLA Yan, Xiao-Yu,et al."Dual-functional OPH-immobilized polyamide nanofibrous membrane for effective organophosphorus toxic agents protection".BIOCHEMICAL ENGINEERING JOURNAL 98.15(2015):47-55.
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