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Ultrasound-assisted aqueous two-phase extraction of phenylethanoid glycosides from Cistanche deserticola Y. C. Ma stems
Dong, Beitao1,2; Yuan, Xiaofan1; Zhao, Qingsheng1; Feng, Qingming3; Liu, Boyan1; Guo, Yuanheng1,2; Zhao, Bing1
2015-04-01
Source PublicationJOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
Volume38Issue:7Pages:1194-1203
AbstractAn efficient ultrasound-assisted aqueous two-phase extraction and enrichment process for phenylethanoid glycosides from Cistanche deserticola Y. C. Ma stems was developed in this work. An ethanol/ammonium sulfate system was chosen for the aqueous two-phase system due to its fine partitioning and recycling behaviors. Single-factorexperiments and response surface methodology were used to optimize the process parameters of the ultrasound-assisted aqueous two-phase extraction. The optimal conditions were as follows: a salt concentration of 23.5%, an ethanol concentration of 20%, an extraction time of 37 min, an extraction temperature of 30 degrees C, a liquid/solid ratio of 30:1 w/w, and an ultrasound power of 300 W. Under the above conditions, the extraction yields of echinacoside and acteoside (the main components of phenylethanoid glycosides) reached 5.35 and 6.22 mg/g dry material weight, respectively. The contents of echinacoside and acteoside in the extracts reached 27.56 and 30.23 mg/g, respectively, which were 2.46- and 2.58-fold higher than the amounts obtained in ultrasound-assisted extraction. In conclusion, ultrasound-assisted aqueous two-phase extraction was an efficient, ecofriendly, and economical method, and it may be a promising technique for extracting and enriching bioactive components from plants.
KeywordActeosides Cistanche Deserticola Echinacosides Phenylethanoid Glycosides
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1002/jssc.201401410
Indexed BySCI
Language英语
WOS KeywordRESPONSE-SURFACE METHODOLOGY ; ANTIOXIDANT ACTIVITY ; SEPARATION ; OLIGOGLYCOSIDES ; CHROMATOGRAPHY ; OPTIMIZATION ; ECHINACOSIDE ; TUBULOSA ; POWDER
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000353053700018
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/16747
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Sch Chem & Chem Engn, Beijing, Peoples R China
3.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Dong, Beitao,Yuan, Xiaofan,Zhao, Qingsheng,et al. Ultrasound-assisted aqueous two-phase extraction of phenylethanoid glycosides from Cistanche deserticola Y. C. Ma stems[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(7):1194-1203.
APA Dong, Beitao.,Yuan, Xiaofan.,Zhao, Qingsheng.,Feng, Qingming.,Liu, Boyan.,...&Zhao, Bing.(2015).Ultrasound-assisted aqueous two-phase extraction of phenylethanoid glycosides from Cistanche deserticola Y. C. Ma stems.JOURNAL OF SEPARATION SCIENCE,38(7),1194-1203.
MLA Dong, Beitao,et al."Ultrasound-assisted aqueous two-phase extraction of phenylethanoid glycosides from Cistanche deserticola Y. C. Ma stems".JOURNAL OF SEPARATION SCIENCE 38.7(2015):1194-1203.
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