CAS OpenIR  > 生化工程国家重点实验室
Tethering of Nicotinamide Adenine Dinucleotide Inside Hollow Nanofibers for High-Yield Synthesis of Methanol from Carbon Dioxide Catalyzed by Coencapsulated Multienzymes
Ji, Xiaoyuan1; Su, Zhiguo1; Wang, Ping1,2,3; Ma, Guanghui1; Zhang, Songping1
2015-04-01
Source PublicationACS NANO
ISSN1936-0851
Volume9Issue:4Pages:4600-4610
Abstract

Enzymatic conversion of carbon dioxide (CO2) to fuel or chemicals is appealing, but is limited by lack of efficient technology for regeneration and reuse of expensive cofactors. Here we show that cationic polyelectrolyte-doped hollow nanofibers, which can be fabricated via a facile coaxial electrospinning technology, provide an ideal scaffold for assembly of cofactor and multienzymes capable of synthesizing methanol from CO2 through a cascade multistep reaction involving cofactor regeneration. Cofactor and four enzymes including formate, formaldehyde, alcohol, and glutamate dehydrogenases were in situ coencapsulated inside the lumen of hollow nanofibers by involving them in the core-phase solution for coaxial electrospinning, in which cationic polyelectrolyte was predissolved. The polyelectrolyte penetrating across the shell of the hollow nanofibers enabled efficient tethering and retention of cofactor inside the lumen via ion-exchange interactions between oppositely charged polyelectrolytes and cofactor. With carbonic anhydrase assembled on the outer surface of the hollow nanofibers for accelerating hydration Of CO2, these five-enzymes-cofactor catalyst system exhibited high activity for methanol synthesis. Compared with methanol yield of only 36.17% using free enzymes and cofactor, the hollow nanofiber-supported system afforded a high value up to 103.2%, the highest reported value so far. It was believed that the linear polyelectrolytes acted as spacers to enhance the shuttling of cofactor between enzymes that were coencapsulated within near vicinity, thus improving the efficiency of the system. The immobilized system showed good stability in reusing. About 80% of its original productivity was retained after 10 reusing cycles, with a cofactor-based cumulative methanol yield reached 940.5%.

KeywordCarbon Dioxide Bioconversion Cofactor Regeneration Hollow Nanofibers Multienzyme System
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
DOI10.1021/acsnano.5b01278
Indexed BySCI
Language英语
WOS KeywordSITU COFACTOR REGENERATION ; GLUCOSE-DEHYDROGENASE ; EFFICIENT CONVERSION ; MEMBRANE REACTOR ; ARTIFICIAL CELLS ; BIENZYME SYSTEM ; CO2 ; REDUCTION ; ANHYDRASE ; ENZYMES
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS IDWOS:000353867000129
Citation statistics
Cited Times:61[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/16770
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA
3.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA
Recommended Citation
GB/T 7714
Ji, Xiaoyuan,Su, Zhiguo,Wang, Ping,et al. Tethering of Nicotinamide Adenine Dinucleotide Inside Hollow Nanofibers for High-Yield Synthesis of Methanol from Carbon Dioxide Catalyzed by Coencapsulated Multienzymes[J]. ACS NANO,2015,9(4):4600-4610.
APA Ji, Xiaoyuan,Su, Zhiguo,Wang, Ping,Ma, Guanghui,&Zhang, Songping.(2015).Tethering of Nicotinamide Adenine Dinucleotide Inside Hollow Nanofibers for High-Yield Synthesis of Methanol from Carbon Dioxide Catalyzed by Coencapsulated Multienzymes.ACS NANO,9(4),4600-4610.
MLA Ji, Xiaoyuan,et al."Tethering of Nicotinamide Adenine Dinucleotide Inside Hollow Nanofibers for High-Yield Synthesis of Methanol from Carbon Dioxide Catalyzed by Coencapsulated Multienzymes".ACS NANO 9.4(2015):4600-4610.
Files in This Item:
File Name/Size DocType Version Access License
Tethering of Nicotin(6863KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ji, Xiaoyuan]'s Articles
[Su, Zhiguo]'s Articles
[Wang, Ping]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ji, Xiaoyuan]'s Articles
[Su, Zhiguo]'s Articles
[Wang, Ping]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ji, Xiaoyuan]'s Articles
[Su, Zhiguo]'s Articles
[Wang, Ping]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.