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Separation of lysozyme from chicken egg white using ultrafiltration
Wan, YH; Lu, JR; Cui, ZF
2006-03-01
Source PublicationSEPARATION AND PURIFICATION TECHNOLOGY
ISSN1383-5866
Volume48Issue:2Pages:133-142
AbstractUsing a methodology based on the combination of recently developed parameter scanning ultrafiltration with carrier phase ultrafiltration (CPUF), this article examines the separation of lysozyme directly from natural chicken egg white (CEW) solutions with 30 kDa membranes. The suitability of two membranes, i.e. Biomax 30 kDa polyethersulfone and Ultracel Amicon YM 30 kDa regenerated cellulose membranes were rapidly assessed and the physicochemical conditions likely to be optimal were identified by parameter scanning ultrafiltration. Under Suitable conditions, with the Biomax 30 kDa membrane, lysozyme transmission from the CEW solution Could be more than 80% and the lysozyme purity obtained in permeate was greater than 94% after one stage Ultrafiltration process. (c) 2005 Elsevier B.V. All rights reserved.
KeywordUltrafiltratiom Lysozyme Physicochemical
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1016/j.seppur.2005.07.003
Indexed BySCI
Language英语
WOS KeywordCARRIER PHASE ULTRAFILTRATION ; FRACTIONATION ; MEMBRANES ; FILTRATION ; PROTEINS ; ALBUMIN ; IGG ; PH
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
WOS IDWOS:000235300500006
Citation statistics
Cited Times:47[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19206
Collection生化工程国家重点实验室
Affiliation1.Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England
2.Chinese Acad Sci, Int Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Wan, YH,Lu, JR,Cui, ZF. Separation of lysozyme from chicken egg white using ultrafiltration[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2006,48(2):133-142.
APA Wan, YH,Lu, JR,&Cui, ZF.(2006).Separation of lysozyme from chicken egg white using ultrafiltration.SEPARATION AND PURIFICATION TECHNOLOGY,48(2),133-142.
MLA Wan, YH,et al."Separation of lysozyme from chicken egg white using ultrafiltration".SEPARATION AND PURIFICATION TECHNOLOGY 48.2(2006):133-142.
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