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In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir.
Guo, Bin; Gao, Min; Liu, Chun-Zhao
2007-03-01
Source PublicationPLANT CELL REPORTS
ISSN0721-7714
Volume26Issue:3Pages:261-265
Abstract

An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and 5.2 shoots per leaf explant were achieved when cultured on a medium containing 10 mu M 6-benzylaminopurine (BAP) and 2.5 mu M 1-naphthaleneacetic acid (NAA). Shoot organogenesis was improved further when the leaf explants were pre-incubated at low temperature, and 80.6% of shoot regeneration frequency was recorded with 9.3 shoots per leaf explant at 4 degrees C by 5-day pretreatment period. Up to 87.0% of the regenerated shoots formed complete plantlets on a medium containing 2.5 mu M indole-3-acetic acid (IAA) within 28 days, and 85.2% of the regenerated plantlets survived and grew vigorously in greenhouse condition. The phytochemical profile of the micropropagated plants was similar to that of wild plants. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of the elite medicinal plant.

KeywordGermplasm Conservation Low Temperature Pretreatment Phytochemicals Saussurea Involucrata Shoot Regeneration
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s00299-006-0230-6
Indexed BySCI
Language英语
WOS KeywordREGENERATION ; TEMPERATURE ; GROWTH
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000244200500002
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19211
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Phytochem Engn Grp, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Guo, Bin,Gao, Min,Liu, Chun-Zhao. In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir.[J]. PLANT CELL REPORTS,2007,26(3):261-265.
APA Guo, Bin,Gao, Min,&Liu, Chun-Zhao.(2007).In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir..PLANT CELL REPORTS,26(3),261-265.
MLA Guo, Bin,et al."In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir.".PLANT CELL REPORTS 26.3(2007):261-265.
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