CAS OpenIR  > 多相复杂系统国家重点实验室
Plant regeneration of an endangered medicinal plant Hydrastis canadensis L.
He, Shan-Shan; Liu, Chun-Zhao; Saxena, Praveen K.
2007-06-05
Source PublicationSCIENTIA HORTICULTURAE
ISSN0304-4238
Volume113Issue:1Pages:82-86
AbstractGoldenseal (Hydrastis canadensis L.) is an endangered medicinal plant used to treat sore eyes and mouths, cold and flu and also as a dye. The objective of this study was to develop an efficient in vitro propagation protocol for goldenseal. Significantly more shoots (26 shoots per leaf explants) were induced on a medium containing 2.5 mu M thidiazuron (TDZ) and 5.0 mu M1-naphthaleneacetic acid (NAA) than any other treatment. Sub-culturing regenerated shoots on a medium with 5.0 mu M 6-benzylaminopurine (BA) induced the maximum rate of shoot multiplication. Growth of the regenerated shoots in a temporary immersion bioreactor resulted in significant increases in biomass, shoot height and shoot multiplication. The regenerated shoots from the temporary immersion bioreactor formed roots when transferred onto a medium with 1.0-2.0 RM indole-3-butyric acid (IBA). Regenerated whole plantlets were acclimatized and maintained in standard greenhouse conditions for further growth. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of this rare, medicinally important species. (C) 2007 Elsevier B.V. All rights reserved.
KeywordBioreactor Culture Germplasm Conservation Goldenseal Hydrastis Canadensis L. Thidiazuron
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.scienta.2007.01.014
Indexed BySCI
Language英语
WOS KeywordARTEMISIA-JUDAICA L ; MICROPROPAGATION ; GOLDENSEAL ; CULTURE ; BIOREACTORS ; CLUSTERS ; GROWTH
WOS Research AreaAgriculture
WOS SubjectHorticulture
WOS IDWOS:000247261700014
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19214
Collection多相复杂系统国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China
2.Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
He, Shan-Shan,Liu, Chun-Zhao,Saxena, Praveen K.. Plant regeneration of an endangered medicinal plant Hydrastis canadensis L.[J]. SCIENTIA HORTICULTURAE,2007,113(1):82-86.
APA He, Shan-Shan,Liu, Chun-Zhao,&Saxena, Praveen K..(2007).Plant regeneration of an endangered medicinal plant Hydrastis canadensis L..SCIENTIA HORTICULTURAE,113(1),82-86.
MLA He, Shan-Shan,et al."Plant regeneration of an endangered medicinal plant Hydrastis canadensis L.".SCIENTIA HORTICULTURAE 113.1(2007):82-86.
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