CAS OpenIR  > 生化工程国家重点实验室
Effect of IPTG amount on apo- and holo- forms of glycerophosphate oxidase expressed in Escherichia coli
Zheng, Chunyang1; Zhao, Zhanyong2; Li, Yan3; Wang, Lin1,2; Su, Zhiguo3
2011-02-01
Source PublicationPROTEIN EXPRESSION AND PURIFICATION
ISSN1046-5928
Volume75Issue:2Pages:133-137
AbstractEscherichia coli has proved to be a successful host for the expression of many heterologous proteins and much efforts have been made toward improving recombinant protein expression including the usage of strong promoters and co-expression with chaperones But little attention was paid on the relation between expression level and function of the target protein Glycerophosphate oxidase (GPO) is a protein with FAD cofactor (without free cysteine and disulfide bonds) It was observed that the specific activity of GPO dramatically decreased with the increase of inducer IPTG In addition the stability of it decreased correspondingly The structural difference of samples expressed under varying IPTG was investigated using size-exclusion and reverse-phase high performance liquid chromatography together with CD spectrum It was found that the conformation of peptide and organization of subunits were not affected The loss of specific activity and stability were correlated to Incomplete attachment of FAD onto GPO These results revealed that synthesis speed should be controlled either by reduction of IPTG amount or using weak promoters in the production of GPO (C) 2010 Elsevier Inc All rights reserved
KeywordApo-gpo Holo Gpo Fad Cofactor Specific Activity
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.pep.2010.08.009
Indexed BySCI
Language英语
WOS KeywordAMINO-ACID OXIDASE ; DIMETHYLGLYCINE DEHYDROGENASE ; 6-HYDROXY-D-NICOTINE OXIDASE ; BACILLUS-SUBTILIS ; COFACTOR BINDING ; GLYCINE OXIDASE
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000285126500003
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19228
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Ctr Struct & Mol Biol, Inst Biophys, Beijing 100101, Peoples R China
2.Biotrand Inc, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zheng, Chunyang,Zhao, Zhanyong,Li, Yan,et al. Effect of IPTG amount on apo- and holo- forms of glycerophosphate oxidase expressed in Escherichia coli[J]. PROTEIN EXPRESSION AND PURIFICATION,2011,75(2):133-137.
APA Zheng, Chunyang,Zhao, Zhanyong,Li, Yan,Wang, Lin,&Su, Zhiguo.(2011).Effect of IPTG amount on apo- and holo- forms of glycerophosphate oxidase expressed in Escherichia coli.PROTEIN EXPRESSION AND PURIFICATION,75(2),133-137.
MLA Zheng, Chunyang,et al."Effect of IPTG amount on apo- and holo- forms of glycerophosphate oxidase expressed in Escherichia coli".PROTEIN EXPRESSION AND PURIFICATION 75.2(2011):133-137.
Files in This Item:
File Name/Size DocType Version Access License
Effect of IPTG amoun(620KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zheng, Chunyang]'s Articles
[Zhao, Zhanyong]'s Articles
[Li, Yan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zheng, Chunyang]'s Articles
[Zhao, Zhanyong]'s Articles
[Li, Yan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zheng, Chunyang]'s Articles
[Zhao, Zhanyong]'s Articles
[Li, Yan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.