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Printed microwells with highly stable thin-film enzyme coatings for point-of-care multiplex bioassay of blood samples
Zhang, Liting1,2; Cao, Xiaodan1; Wang, Lu2; Zhao, Xueyan2; Zhang, Songping3; Wang, Ping2
2015
Source PublicationANALYST
ISSN0003-2654
Volume140Issue:12Pages:4105-4113
Abstract

A paper-based colorimetric biosensor suitable for point-of-care bioassay of blood samples is developed using highly stable enzyme thin-film coatings confined within inkjet printed polymeric microwells. The microwells are developed through a simple one-step inkjet printing of hydrophobic polystyrene on paper, with walls formed by the polymer that fills the gaps inside the paper body. The microwells can also be patterned to be interlinked with printed microchannels for multiplex bioassays. Thin film enzyme coatings confined within the microwells are then constructed, thereby constituting biosensors that work like traditional microwell plates, yet allow easy colorimetric readouts with naked eyes or portable devices, such as smart phones. The efficiency of the paper-based sensor was demonstrated for colorimetric assays of glucose and lactate, both as individual analytes or mixed, as well as samples with red blood cells. Such sensors showed good sensitivities within the concentration ranges of the analytes in human blood (0.5-10 mM), with a visible sensitivity of < 0.5 mM detectable by naked eyes for a sample size as small as 1 mu L. More accurate digital readouts were shown to be feasible with computerized scanners or smart-phones. The thin-film coating format affords the paper biosensors an extended lifetime, and they could retain 100% performance over 6 months of storage at room temperature, or up to one month heated at 50 degrees C, which promises refrigeration-free storage of the sensor. The simple preparation, high enzyme stability and ease-of-use of the paper-based sensor promise low-cost and reliable point-of-care multiplex bioassay for biomedical diagnostics.

SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1039/c5an00054h
Indexed BySCI
Language英语
WOS KeywordPAPER-BASED MICROFLUIDICS ; GLUCOSE SENSOR ; DIAGNOSTICS ; SMARTPHONE
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000355852400029
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19263
Collection生化工程国家重点实验室
Affiliation1.E China Univ Sci & Technol, Biomed Nanotechnol Ctr, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
2.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Liting,Cao, Xiaodan,Wang, Lu,et al. Printed microwells with highly stable thin-film enzyme coatings for point-of-care multiplex bioassay of blood samples[J]. ANALYST,2015,140(12):4105-4113.
APA Zhang, Liting,Cao, Xiaodan,Wang, Lu,Zhao, Xueyan,Zhang, Songping,&Wang, Ping.(2015).Printed microwells with highly stable thin-film enzyme coatings for point-of-care multiplex bioassay of blood samples.ANALYST,140(12),4105-4113.
MLA Zhang, Liting,et al."Printed microwells with highly stable thin-film enzyme coatings for point-of-care multiplex bioassay of blood samples".ANALYST 140.12(2015):4105-4113.
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