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Bare magnetic nanoparticles as fluorescence quenchers for detection of thrombin
Yu, Jiemiao1,2,3; Yang, Liangrong2; Liang, Xiangfeng2; Dong, Tingting2,3; Liu, Huizhou1,2
2015
Source PublicationANALYST
ISSN0003-2654
Volume140Issue:12Pages:4114-4120
AbstractRapid and sensitive detection of thrombin has very important significance in clinical diagnosis. In this work, bare magnetic iron oxide nanoparticles (magnetic nanoparticles) without any modification were used as fluorescence quenchers. In the absence of thrombin, a fluorescent dye (CY3) labeled thrombin aptamer (named CY3-aptamer) was adsorbed on the surface of magnetic nanoparticles through interaction between a phosphate backbone of the CY3-aptamer and hydroxyl groups on the bare magnetic nanoparticles in binding solution, leading to fluorescence quenching. Once thrombin was introduced, the CY3-aptamer formed a G-quartet structure and combined with thrombin, which resulted in the CY3-aptamer being separated from the magnetic nanoparticles and restoration of fluorescence. This proposed assay took advantage of binding affinity between the CY3-aptamer and thrombin for specificity, and bare magnetic nanoparticles for fluorescence quenching. The fluorescence signal had a good linear relationship with thrombin concentration in the range of 1-60 nM, and the limit of detection for thrombin was estimated as low as 0.5 nM. Furthermore, this method could be applied for other target detection using the corresponding fluorescence labeled aptamer.
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1039/c5an00519a
Indexed BySCI
Language英语
WOS KeywordHOST-GUEST-RECOGNITION ; SINGLE-STRANDED-DNA ; ELECTROCHEMICAL APTASENSOR ; MASS-SPECTROMETRY ; GOLD NANOPARTICLES ; ULTRASENSITIVE DETECTION ; SIGNAL AMPLIFICATION ; OXIDE NANOPARTICLES ; SENSITIVE DETECTION ; GRAPHENE OXIDE
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000355852400030
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19268
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yu, Jiemiao,Yang, Liangrong,Liang, Xiangfeng,et al. Bare magnetic nanoparticles as fluorescence quenchers for detection of thrombin[J]. ANALYST,2015,140(12):4114-4120.
APA Yu, Jiemiao,Yang, Liangrong,Liang, Xiangfeng,Dong, Tingting,&Liu, Huizhou.(2015).Bare magnetic nanoparticles as fluorescence quenchers for detection of thrombin.ANALYST,140(12),4114-4120.
MLA Yu, Jiemiao,et al."Bare magnetic nanoparticles as fluorescence quenchers for detection of thrombin".ANALYST 140.12(2015):4114-4120.
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