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A hydrophobic interaction chromatography strategy for purification of inactivated foot-and-mouth disease virus
Li, Hao1,2; Yang, Yanli1,2; Zhang, Yan1; Zhang, Songping1; Zhao, Qizu3; Zhu, Yuanyuan3; Zou, Xingqi3; Yu, Mengran1,2; Ma, Guanghui1; Su, Zhiguo1
2015-09-01
Source PublicationPROTEIN EXPRESSION AND PURIFICATION
ISSN1046-5928
Volume113Issue:SEPPages:23-29
AbstractA purification scheme based on hydrophobic interaction chromatography was developed to separate inactivated foot-and-mouth disease virus (FMDV) from crude supernatant. About 92% recovery and 8.8-fold purification were achieved on Butyl Sepharose 4FF. Further purification on Superdex 200 resulted in another 29-fold purification, with 92% recovery. The columns were coupled through an intermediate ultrafiltration unit to concentrate the virus. The entire process was completed in about 3.5 h, with 75% final FMDV recovery, and 247-fold purification. The final product had purity above 98%, with over 99.5% of host cell DNA removed. High-performance size exclusion chromatography (HPSEC), Western blot, dynamic light scattering (DLS), and transmission electron microscopy (TEM) indicated that the purified virus contained the required antigen, and was structurally intact with a spherical shape and a particle size of 28 nm. (C) 2015 Elsevier Inc. All rights reserved.
KeywordFoot-and-mouth Disease Virus Hydrophobic Interaction Chromatography Purification Downstream Processing
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.pep.2015.04.011
Indexed BySCI
Language英语
WOS KeywordSIZE-EXCLUSION CHROMATOGRAPHY ; ION-EXCHANGE CHROMATOGRAPHY ; B SURFACE-ANTIGEN ; HANSENULA-POLYMORPHA ; PARTICLES ; VACCINES ; QUANTIFICATION ; CAPSIDS ; FMDV ; CELL
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000356113300004
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19297
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.China Inst Vet Drug Control, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Li, Hao,Yang, Yanli,Zhang, Yan,et al. A hydrophobic interaction chromatography strategy for purification of inactivated foot-and-mouth disease virus[J]. PROTEIN EXPRESSION AND PURIFICATION,2015,113(SEP):23-29.
APA Li, Hao.,Yang, Yanli.,Zhang, Yan.,Zhang, Songping.,Zhao, Qizu.,...&Su, Zhiguo.(2015).A hydrophobic interaction chromatography strategy for purification of inactivated foot-and-mouth disease virus.PROTEIN EXPRESSION AND PURIFICATION,113(SEP),23-29.
MLA Li, Hao,et al."A hydrophobic interaction chromatography strategy for purification of inactivated foot-and-mouth disease virus".PROTEIN EXPRESSION AND PURIFICATION 113.SEP(2015):23-29.
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